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Viral polymerases and related proteins
Title
Viral polymerases and related proteins / edited by Lawrence C. Kuo, David B. Olsen, Steven S. Carroll.
ISBN
0121821765
9780121821760
Published
San Diego : Academic Press, ©1996.
Physical Description
1 online resource (xxx, 658 pages) : illustrations.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * Expression, purification, and characterization * Activity assays * Kinetic and screening * Design and analysis of substrates and inhibitors * Molecular and structural characterizations.
Variant and related titles
Elsevier ScienceDirect All Books. OCLC KB.
Other formats
Print version: Viral polymerases and related proteins. San Diego : Academic Press, ©1996
Format
Books / Online
Language
English
Added to Catalog
May 17, 2018
Series
Methods in enzymology, v. 275
Bibliography
Includes bibliographical references and indexes.
Partial contents
Sect. 1. Expression, purification, and characterization
Purification of viral polymerases: general considerations
Expression, purification, and characterization of the herpes simplex virus type-1 DNA polymerase
Assays for poliovirus polymerase, 3Dpol, and authentic RNA replication in HeLa S10 extracts
RNA-dependent RNA polymerase of hepatitis C virus
Characterization of coronavirus RNA polymerase gene products
Expression, purification, and characterization of orthomyxovirus: influenza transcriptase
Expression, purification, and characterization of rhabdovirus polymerase
Expression and purification of retroviral HIV-1 reverse transcriptase
Expression, purification, and characterization of DNA polymerases involved in papovavirus replication
Heterologous expression, purification, and characterization of adenovirus DNA polymerase and preterminal protein
Expression and characterization of hepadnavirus reverse transcriptases
Expression, purification, and characterization of vaccinia virus-encoded RNA and poly(A)polymerases
Viral polymerase-associated 5'(arrow)3' exonucleases: expression, purification, and uses
Sect. 2. Activity assays: kinetic and screening
Isotopic assays of viral polymerases and related proteins
Nonisotopic assays of viral polymerases and related proteins
Catalytic activities associated with retroviral and viral polymerases
Approaches to high-volume screening assays of viral polymerases and related proteins
In situ DNA polymerase and RNase H activity gel assays as applied to hepadnavirus particles
A system to analyze and identify inhibitors of HIV-1 gene regulation using a defective integrated provirus
Sect. 3. Design and analysis of substrates and inhibitors
Preparation and use of synthetic oligoribonucleotides as tools for study of viral polymerases
Use of bacteriophage RNA polymerase in RNA synthesis
Inhibition of viral polymreases by chain-terminating substrates: a kinetic analysis
Design of nucleoside analog inhibitors of herpes-virus polymerases
Development of nonucleoside HIV reverse transcriptase inhibitors
Analysis of inhibition of retroviral reverse transcriptase
Novel methods of generating specific oligonucleotide inhibitors of viral polymerases
Sect. 4. Molecular and structural characterizations
Analyzing the fidelity of reverse transcription and transcription
Site-directed mutagenic analysis of viral polymerases and related proteins
Analysis of resistance mutants of viral polymerases
Author index
Subject index.
Subjects (Medical)
DNA, Viral - physiology.
DNA-Directed DNA Polymerase - isolation & purification.
DNA-Directed RNA Polymerases - isolation & purification.
Genre/Form
Electronic books.
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