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Cryo-EM. Part A, Sample preparation and data collection
Title
Cryo-EM. Part A, Sample preparation and data collection / edited by Grant J. Jensen.
ISBN
9780080956954
0080956955
9780123749062
0123749069
Published
San Diego, Calif : Academic Press/Elsevier, ©2010.
Physical Description
1 online resource (xlv, 410 pages) : illustrations.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, intrumentation, and methods underlying all cryo-EM approaches which allow practictioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. * Includes time-tested core methods and new innovations applicable to any researcher * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide.
Variant and related titles
Elsevier ScienceDirect All Books. OCLC KB.
Other formats
Print version: Sample preparation and data collection. London : Academic, 2010
Format
Books / Online
Language
English
Added to Catalog
May 17, 2018
Series
Methods in enzymology, v. 481
Bibliography
Includes bibliographical references and indexes.
Contents
Part A: Sample preparation and data collection. 3d reconstruction from electron micrographs a personal account of its development
Preparation of 2d crystals of membrane proteins for high-resolution electron crystallography data collection
Helical crystallization of soluble and membrane binding proteins
Plunge freezing for electron cryomicroscopy
A practical guide to the use of monolayer purification and affinity grids
Grafix: Stabilization of fragile macromolecular complexes for single particle cryo-EM
Cryonegative staining of macromolecular assemblies
Liposomes on a streptavidin crystal: A system to study membrane proteins by cryo-EM
Micromanipulator-assisted vitreous cryosectioning and sample preparation by high-pressure freezing
Site-specific biomolecule labeling with gold clusters
How to operate a cryo-electron microscope
Collecting electron crystallographic data of two-dimensional protein crystals
Automated data collection for electron microscopic tomography
Correlated light and electron cryo-microscopy
Phase plates for transmission electron microscopy
Radiation damage in electron cryomicroscopy.
Subjects
Subjects (Medical)
Microscopy, Electron.
Genre/Form
Electronic books.
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