9780128005958

0128005955

9780128004531

0128004533

Advances in Protein Chemistry and Structural Biology, Volume 95

Front Cover; Proteomics in Biomedicine and Pharmacology; Copyright; Contents; Contributors; Preface; Chapter One: Application of Cutting-Edge Proteomics Technologies for Elucidating Host-Bacteria Interactions; 1. Introduction; 2. Classical Proteomics Strategies for Biomedical Research in General; 2.1. Gel-based methods; 3. Gel-Free Methods; 3.1. Gel-free-labeling methods; 3.1.1. Chemical labeling; 3.1.2. Metabolic labeling; 3.2. Label-free and absolute quantification; 4. New Proteomic Methods in Looking for Bacterial Pathogens; 5. Proteomic Advances in Looking for Host Organisms; 6. Prospects.

3. Applications of Phosphoproteomics in Biomedicine3.1. Applications in cancer research; 3.2. Applications in stem cell research; 3.3. Applications in cardiac research; 3.4. Applications in immunity research; 4. Discussion; References; Chapter Three: Recent Advances in Mass Spectrometry-Based Glycoproteomics; 1. Introduction; 2. Glycoproteomic Profiling by MS; 2.1. Glycoproteomics methodology; 2.2. Affinity enrichment; 2.3. Glycoprotein digestion; 2.4. Glycan release; 2.5. Chromatographic separation and SPE; 2.6. Mass spectrometry; 2.7. Quantitation; 2.8. Bioinformatics.

3. MS-Based Glycoproteomics in Disease Research3.1. Cancer biomarker research; 3.2. Neurodegenerative disease research; 4. Concluding Remarks; Acknowledgments; References; Chapter Four: Proteomics and Proteogenomics Approaches for Oral Diseases; 1. Introduction; 2. An Integrated Proteogenomics Protocol for Personalized Dentistry; 2.1. Human samples; 2.1.1. Tissues; 2.1.2. Blood; 2.1.3. Dental plaque and oral biofilms; 2.1.4. Gingival crevicular fluid; 2.1.5. Saliva; 2.1.6. Oral rinse; 2.2. Bioinformatics analysis; 2.2.1. Bioinformatics resources; 2.2.2. Leader-gene algorithm.

2.3. Proteomics technologies, with a focus on the label-free tools2.3.1. Mass spectrometry; 2.3.2. Anodic porous alumina; 2.3.3. Nucleic acid programmable protein arrays; 3. Oral Diseases; 3.1. Dental caries; 3.2. Periodontitis; 3.3. Oral lichen planus; 3.4. Oral cancer; 4. Concluding Remarks; References; Chapter Five: Advances in Nanocrystallography as a Proteomic Tool; 1. Introduction; 2. Langmuir-Blodgett (LB)-Based Crystallization; 3. Comparison of LB-Based Crystallization with Other Techniques; 4. Fourier Transform Infrared (FTIR) Spectroscopy for Investigating LB-Films.