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Ion Channel Localization Methods and Protocols

Title
Ion Channel Localization [electronic resource] : Methods and Protocols / edited by Anatoli N. Lopatin, Colin G. Nichols.
ISBN
9781592591183
Publication
Totowa, NJ : Humana Press, 2001.
Physical Description
1 online resource (XVII, 502 p.)
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
Although there are many useful books on virtually every aspect of ion channel structure and function, work on channel localization-determining the specific sites of ion channels and their receptors on cells-has long been neglected. In Ion Channel Localization: Methods and Protocols, active researchers at the cutting-edge of their fields describe how to use current and developing technologies to determine the exact cell sites of such channels and receptors. With emphasis on techniques employing the unique properties of these proteins, the core topics of the book include all the major pharmacological tools for ion channel determination. Also covered are fluorescent and radioligand assays, exciting new visualization methods employing green fluorescent protein (GFP), and novel assays based on the functional properties of ion channels and receptors, as well as rapidly developing atomic force microscopy methodologies. Comprehensive and rich in practical detail, Ion Channel Localization: Methods and Protocols brings together in a single volume detailed reviews of all the major techniques-both currently in use and in development-for localizing ion channels and receptors in cells, tissues, and organisms.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
August 31, 2018
Series
Methods in Pharmacology and Toxicology,
Methods in Pharmacology and Toxicology,
Contents
Pharmacological Labeling of Ion Channels and Receptors
Fluorescent Calcium Antagonists Tools for Imaging of L-Type Calcium Channels in Living Cells
Targeting Cerebral Muscarinic Acetylcholine Receptors with Radioligands for Diagnostic Nuclear Medicine Studies
GABAA Receptors
Localization of ATP P2X Receptors
Small Conductance Calcium-Activated Potassium Channels in Rat Brain
Mapping N-Type Calcium Channel Distributions with H-Conotoxins
Fluorescent Imaging of Nicotinic Receptors During Neuromuscular Junction Development
High Affinity Scorpion Toxins for Studying Potassium and Sodium Channels
Applications of Green Fluorescent Protein (GFP) Technology
Bicistronic GFP Expression Vectors as a Tool to Study Ion Channels in Transiently Transfected Cultured Cells
Confocal Imaging of GFP-Labeled Ion Channels
Localization and Quantification of GFP-Tagged Ion Channels Expressed in Xenopus Oocytes
Applications of Green Fluorescent Protein (GFP) Technology
Tagging Ion Channels with the Green Fluorescent Protein (GFP) as a Method for Studying Ion Channel Function in Transgenic Mouse Models
Mutant GFP- Based FRET Analysis of K+ Channel Organization
Delivering Ion Channels to Mammalian Cells by Membrane Fusion
Functional Assays in Ion Channel Localization
Detection of Neurons Expressing Calcium- Permeable AMPA Receptors Using Kainate-Induced Cobalt Uptake
Crystallization Technique for Localizing Ion Channels in Living Cells
Imaging of Localized Neuronal Calcium Influx
Using Caged Compounds to Map Functional Neurotransmitter Receptors
Atomic Force Microscopy
Atomic Force Microscopy of Reconstituted Ion Channels
Imaging the Spatial Organization of Calcium Channels in Nerve Terminals Using Atomic Force Microscopy
Nanoarchitecture of Plasma Membrane Visualized with Atomic Force Microscopy
Dynamic AFM of Patch Clamped Membranes
Localizing Ion Channels with Scanning Probe Microscopes: A Perspective.
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