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Cancer Gene Profiling Methods and Protocols

Title
Cancer Gene Profiling [electronic resource] : Methods and Protocols / edited by Robert Grützmann, Christian Pilarsky.
ISBN
9781597455459
Publication
Totowa, NJ : Humana Press, 2010.
Physical Description
1 online resource (XII, 452 p.)
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
Over the last few years, the methods for analyzing cancer-related genes on a molecular level have changed rapidly. The advent of incredible new technology, such as automated sequencing, has served as a catalyst for many advances in the area of gene profiling, and has enabled the research community to investigate populations of cells in a given tissue. Cancer Gene Profiling: Methods and Protocols examines the world of cancer gene profiling in light of these new advances, providing highly developed microarray techniques alongside modern methods for arraying tissues and proteomics. Chapters bring together the experiences of leading scientists in each method of profiling, providing a clear overview of the ground-breaking techniques used to perform these experiments effectively. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Cancer Gene Profiling: Methods and Protocols is a fundamental guidebook for researchers in the field of cancer gene profiling, as well as for those individuals in the area of biomedical/translational research.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
August 31, 2018
Series
Methods in Molecular Biology, Methods and Protocols, 576
Methods in Molecular Biology, Methods and Protocols, 576
Contents
Organizational Issues in Providing High-Quality Human Tissues and Clinical Information for the Support of Biomedical Research
Manual Microdissection
Laser Microdissection
Tissue Microarrays
A Decade of Cancer Gene Profiling: From Molecular Portraits to Molecular Function
Mining Expressed Sequence Tag (EST) Libraries for Cancer-Associated Genes
Automated Fluorescent Differential Display for Cancer Gene Profiling
Manual Microdissection Combined with Antisense RNA–LongSAGE for the Analysis of Limited Cell Numbers
Quantitative DNA Methylation Profiling on a High-Density Oligonucleotide Microarray
Single-Nucleotide Polymorphism (SNP) Analysis to Associate Cancer Risk
Application of Proteomics in Cancer Gene Profiling: Two-Dimensional Difference in Gel Electrophoresis (2D-DIGE)
Search for and Identification of Novel Tumor-Associated Autoantigens
Integrative Oncogenomic Analysis of Microarray Data in Hematologic Malignancies
Cancer Gene Profiling in Pancreatic Cancer
Cancer Gene Profiling in Prostate Cancer
Cancer Gene Profiling for Response Prediction
The EGFR Pathway as an Example for Genotype: Phenotype Correlation in Tumor Genes
Quantitation of CD39 Gene Expression in Pancreatic Tissue by Real-Time Polymerase Chain Reaction
Functional Profiling Methods in Cancer
Calibration of Microarray Gene-Expression Data
Meta-analysis of Cancer Gene-Profiling Data
Target Gene Discovery for Novel Therapeutic Agents in Cancer Treatment.
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