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cDNA Libraries Methods and Applications
Title
cDNA Libraries [electronic resource] : Methods and Applications / edited by Chaofu Lu, John Browse, James G. Wallis.
ISBN
9781617790652
Publication
Totowa, NJ : Humana Press, 2011.
Physical Description
1 online resource (XI, 275 p.)
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
The numerous vital applications of complementary DNA (cDNA) technology have changed dramatically as the technology has advanced over recent years. In cDNA Libraries: Methods and Protocols, expert researchers provide current techniques that reflect the latest advances in the construction and application of cDNA libraries. The first half of the volume covers improved approaches to some of the most basic elements of creating cDNA libraries, while the second half casts a much wider net and includes visionary applications of cDNA technology which were either unforeseen or technically impractical until recently. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, cDNA Libraries: Methods and Protocols serves as an ideal guide to all scientists seeking to advance this important technology and provide answers to the enduring fundamental questions of biology.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
August 31, 2018
Series
Methods in Molecular Biology, Methods and Protocols, 729
Contents
Isolation of Fungal Infection Structures from Plant Tissue by Flow Cytometry for Cell-Specific Transcriptome Analysis
Preparation of a High Quality cDNA Library from a Single-Cell Quantity of mRNA Using Chum-RNA
Construction of a Full-Length cDNA Library from Castor Endosperm for High-throughput Functional Screening
Full-Length Transcriptome Analysis Using a Bias-Free cDNA Library Prepared with the Vector-Capping Method
Construction of Improved Yeast Two-Hybrid Libraries
Normalization of Full-Length Enriched cDNA
Bioinformatic Methods for Finding Differentially Expressed Genes in cDNA Libraries, Applied to the Identification of Tumour Vascular Targets
Enzymatic Production of RNAi Libraries from cDNAs and High-Throughput Selection of Effective shRNA Expression Constructs
Construction of Small RNA cDNA Libraries for High-Throughput Sequencing
Focusing Mutations Within Random Libraries to Distinct Areas: Protein Domain Library Generation by Overlap Extension (PDLGO)
Generation of Families of Construct Variants Using Golden Gate Shuffling
Application of Full-Length cDNA Resources to Gain-of-Function Technology for Characterization of Plant Gene Function
Construction of Yeast Surface-Displayed cDNA Libraries
Identification of Protein/Target Molecule Interactions Using Yeast Surface-Displayed cDNA Libraries
SNP Discovery by Transcriptome Pyrosequencing
RNA-seq Analysis of Gene Expression and Alternative Splicing by Double-Random Priming Strategy
Generation of a Large Catalog of Unique Transcripts for Whole-Genome Expression Analysis in Non-Model Species.
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