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Molecular Chaperones Methods and Protocols
Title
Molecular Chaperones [electronic resource] : Methods and Protocols / edited by Stuart K. Calderwood, Thomas L. Prince.
ISBN
9781617792953
Publication
Totowa, NJ : Humana Press, 2011.
Physical Description
1 online resource (XVIII, 320 p.) 42 illus.
Local Notes
Access is available to the Yale community.
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Access restricted by licensing agreement.
Summary
The proteome consists of a complex mixture of proteins each of which need to be folded correctly in order to function for the health of the organism, and many of these proteins require molecular chaperones to reach the correct conformation and, in some cases, to remain in a folded form. In Molecular Chaperones: Methods and Protocols, expert researchers address a wide variety of approaches to the study these mechanisms, featuring the workings of heat shock proteins and heat shock transcription factors, in vitro and in vivo. Written in the highly successful Methods in Molecular Biology™ series format, chapters features introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Molecular Chaperones: Methods and Protocols serves as an ideal guide for all scientists who wish to pursue this vital biological action and its impact on human health and disease.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
August 31, 2018
Series
Methods in Molecular Biology, Methods and Protocols, 787
Contents
Targeted Deletion of Hsf1, 2, and 4 Genes in Mice
The Role Of Heat Shock Factors in Stress-Induced Transcription
Hsp90 and Client Protein Maturation.- The Role of p23, Hop, Immunophilins, and Other Co-Chaperones in Regulating Hsp90 Function
Detecting Hsp90 Phosphorylation
Role of Molecular Chaperones in Biogenesis of the Protein Kinome
Nucleotide Exchange Factors for Hsp70 Chaperones
Reconstitution of CHIP E3 Ubiquitin Ligase Activity
Structure-Functions of HspB1 (Hsp27)
Combined Lentiviral and RNAi Technologies for the Delivery and Permanently Silencing of the hsp25 Gene
Quantification of HSP27 and HSP70 Molecular Chaperone Activities
Measuring Hsp72 (HSPA1A) by Indirect Sandwich ELISA
Analysis of Heat Shock Protein Localization Using Flow Cytometry
Quantitation of Heat Shock Proteins in Clinical Samples Using Mass Spectrometry
Bioinformatic Approach to Identify Chaperone Pathway Relationship from Large-Scale Interaction Networks
Hsp70: Anti-Apoptotic and Tumorigenic Protein
Determination of Cell Survival or Death
Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer
Preparation of a Heat Shock Proteins 70-Based Vaccine from DC-Tumor Fusion Cells
Isolation of Heat Shock Protein Complexes
Enhancing Antigen Cross-Presentation and T-Cell Priming by Complexing Protein Antigen to Recombinant Large Heat Shock Protein
Investigating Receptors for Extracellular Heat Shock Proteins
Analysis of Cellular Migration Using a Two-Chamber Methodology.
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