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G Protein-Coupled Receptor Signaling in Plants Methods and Protocols
Title
G Protein-Coupled Receptor Signaling in Plants [electronic resource] : Methods and Protocols / edited by Mark P. Running.
ISBN
9781627035323
Publication
Totowa, NJ : Humana Press : Imprint: Humana Press, 2013.
Physical Description
1 online resource (X, 162 p.) 19 illus., 14 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
Remarkably, while G protein-coupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols, experts in the field describe techniques used in the study of small GTPases and related proteins. Beginning with a chapter on bioinformatics approaches for GPCR discovery, this detailed volume continues with chapters on heterotrimeric G protein subunits, Rab-GTPases, as well as lipid modifications, including myristoylation, acylation, and prenylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and dependable, G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols aims to aid further studies into the roles of small GTPases which will help elucidate numerous key processes in plants.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
August 31, 2018
Series
Methods in Molecular Biology, Methods and Protocols, 1043
Contents
Topology Assessment, G Protein-Coupled Receptor (GPCR) Prediction, and In Vivo Interaction Assays to Identify Plant Candidate GPCRs
Measurement of GTP-Binding and GTPase Activity of Heterotrimeric Gα Proteins
Biochemical Analysis of the Interaction Between Phospholipase Dα1 and GTP-Binding Protein α-Subunit from Arabidopsis thaliana
Analysis of Cell Division and Cell Elongation in the Hypocotyls of Arabidopsis Heterotrimeric G Protein Mutants
Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G Proteins in Arabidopsis
Methods for Analysis of Disease Resistance and the Defense Response in Arabidopsis
Fusarium oxysporum Infection Assays in Arabidopsis
Analysis of Unfolded Protein Response in Arabidopsis
Functional Analysis of Heterotrimeric G Proteins in Chloroplast Development in Arabidopsis
G Protein Signaling in UV Protection: Methods for Understanding the Signals in Young Etiolated Seedlings
Functional Analysis of Small Rab GTPases in Cytokinesis in Arabidopsis thaliana
In Vivo Localization in Arabidopsis Protoplasts and Root Tissue
Analysis of Protein Prenylation and S-Acylation Using Gas Chromatography–Coupled Mass Spectrometry
In Vitro Myristoylation Assay of Arabidopsis Proteins
Assaying Protein S-Acylation in PlantIn Vitro Prenylation Assay of Arabidopsis Proteins.
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