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Bioluminescent Imaging Methods and Protocols
Title
Bioluminescent Imaging [electronic resource] : Methods and Protocols / edited by Christian E. Badr.
ISBN
9781627037181
Publication
Totowa, NJ : Humana Press : Imprint: Humana Press, 2014.
Physical Description
1 online resource (XI, 276 p.) 50 illus., 39 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
Bioluminescent Imaging: Methods and Protocols distills a wide range of techniques that use bioluminescence imaging as a tool for visualizing and tracking various biological processes. Covering diverse fields such as cellular and molecular biology, oncology, neurology, infectious diseases, immunology, and others, the detailed chapters of this volume are arranged by topic and describe practical procedures and applications of different bioluminescent reporters, from photoproteins (Aequorin) to bacterial luciferases as well as other secreted (such as Gaussia) and non-secreted luciferases (such as Firefly). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips for troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols aims to provide diverse and comprehensive techniques to researchers interested in implementing bioluminescence-based imaging in their laboratory, regardless of their previous level of experience with such methodologies.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
August 31, 2018
Series
Methods in Molecular Biology, Methods and Protocols, 1098
Contents
Bioluminescence Imaging: Basics and Practical Limitations
Extraction and Quantification of Adenosine Triphosphate in Mammalian Tissues and Cells
Neuronal Network Imaging in Acute Slices Using Ca2+ Sensitive Bioluminescent Reporter
Gaussia Luciferase-Based Mycoplasma Detection Assay in Mammalian Cell Culture
Split Gaussia Luciferase for Imaging Ligand-Receptor Binding
Video-Rate Bioluminescence Imaging of Protein Secretion from a Living Cell
Bioluminescence Reporter Gene-Based Detection of MicroRNAs
Monitoring of Transcriptional Dynamics of HIF and NFκB Activities
Real-Time Bioluminescent Tracking of Cellular Population Dynamics
Fabrication of Bioluminescent Capsules and Live-Cell Imaging
The Bioluminescent Imaging of Spontaneously Occurring Tumors in Immunocompetent ODD-Luciferase Bearing Transgenic Mice
Blood-Based Assay with Secreted Gaussia Luciferase to Monitor Tumor Metastasis
Bioluminescence Imaging of Fungal Biofilm Development in Live Animals
Bioluminescent Imaging of Bacteria During Mouse Infection
Cell-Based Bioluminescence Screening Assays
Bioluminescence-Based Monitoring of Virus Vector-Mediated Gene Transfer in Mice
Simultaneous In Vivo Monitoring of Regulatory and Effector T Lymphocytes Using Secreted Gaussia Luciferase, Firefly Luciferase, and Secreted Alkaline Phosphatase
Multiplex Functional Bioluminescent Reporters Using Gaussia Luciferase Fused to Epitope Tags in an Immunobinding Assay
Non-Invasive In Vivo Monitoring of Extracellular Vesicles
In Vitro and In Vivo Demonstrations of Fluorescence by Unbound Excitation from Luminescence (FUEL).
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