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T-Cell Development Methods and Protocols
Title
T-Cell Development [electronic resource] : Methods and Protocols / edited by Rémy Bosselut, Melanie S. Vacchio.
ISBN
9781493928095
Edition
1st ed. 2015.
Publication
New York, NY : Springer New York : Imprint: Humana Press, 2016.
Physical Description
1 online resource (XI, 297 p.) 52 illus., 24 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume provides simple and accessible experiment protocols to explore thymus biology. T-Cell Development: Methods and Protocols is divided into three parts presenting short reviews on T cell development, analysis strategies, protocols for cell preparation, flow cytometry analyses, and multiple aspects of thymocyte biology. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, T-Cell Development: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
August 31, 2018
Series
Methods in Molecular Biology, 1323
Contents
200 Million Thymocytes and I: A Beginner’s Survival Guide to T cell Development
Development of gd T Cells, the Special Forces Soldiers of the Immune System
Genetic Tools to Study T Cell Development
Assessment of T cell Development by Flow Cytometry
Flow Cytometry Analysis of Thymic Cells and their Subpopulations
Identifying the Spatial Relationships of Thymic Stromal and Thymocyte Subsets by Immunofluorescence Analysis
Purification of Thymocyte and T Cell Subsets
Retroviral Transduction of T Cells and T Cell Precursors
Bone Marrow and Fetal Liver Radiation Chimeras
In vitro Analyses of T Cell Effector Differentiation
Studying T Cell Development in Thymic Slices
FTOC-based Analysis of Negative Selection
Reconstituted Thymus Organ Culture
Induction of T-cell Development in vitro by Delta-like (Dll)-Expressing Stromal Cells
In vitro Analysis of Thymocyte Signaling
Molecular Analysis of Mouse T Cell Receptor a and b Gene Rearrangements
Intrathymic Injection
Analysis of Cell Proliferation and |Homeostasis using EdU Labeling
Characterization and Isolation of Human T Cell Progenitors
Approaches to Study Human T Cell Development
Humanized Mice to Study Human T Cell Development
Using the Zebrafish Model to Study T cell Development.
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