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T-Cell Trafficking Methods and Protocols
Title
T-Cell Trafficking [electronic resource] : Methods and Protocols / edited by George Edward Rainger, Helen M. Mcgettrick.
ISBN
9781493969319
Edition
2nd ed. 2017.
Publication
New York, NY : Springer New York : Imprint: Humana Press, 2017.
Physical Description
1 online resource (XI, 252 p.) 47 illus., 32 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This second edition provides updated and new chapters on T-Cell trafficking. In addition to detailed experimental procedures, the interested reader will find informative introductory chapters on the relevance of T-Cell trafficking in thymic population and maturation, traffic through secondary lymphoid organs during ‘physiological’ resolving inflammation and during immune responses, as well as T-Cell trafficking in chronic inflammatory diseases. Importantly, chapters cover methods from in silico modeling of cellular interactions, in vitro adhesion assays, through ex vivo functional assays to integrated intravital modeling of T-Cell trafficking through organs. Written in the highly successful Methods in Molecular Biology series format, each methods chapter includes a short introduction to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, T-Cell Trafficking: Methods and Protocols, Second Edition aims to be an essential point of reference for those new to the field of T-Cell trafficking, or to those looking to expand their technical capabilities.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
August 31, 2018
Series
Methods in Molecular Biology, 1591
Contents
Introduction to Homeostatic Migration
Analysis of Thymocyte Migration, Cellular Interactions, and Activation by Multi-Photon Fluorescence Microscopy of Live Thymic Slices
Visualising and Tracking T Cell Motility in vivo
Graph Theory-based Analysis of the Lymph Node Fibroblastic Reticular Cell Network
Introduction: T cell Trafficking in Inflammation and Immunity
Leukocyte Adhesion under Haemodynamic Flow Conditions
Endocrine Regulation of Lymphocyte Trafficking In Vitro
Mesenchymal Stromal Cells as Active Regulators of Lymphocyte Recruitment to Blood Vascular Endothelial Cells
Monitoring RhoGTPase Activity in Leukocytes using Classic “pull-down” Assays
Utilising Lentiviral Gene Transfer in Primary Endothelial Cells to Assess Lymphocyte-Endothelial Interactions
Introduction to Lymphocyte Trafficking in Disease
Using Ex-Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking
In vitro and Ex Vivo Models to Study T Cell Migration through the Human Liver Parenchyma
Monitoring Migration of Activated T Cells to Antigen-rich Non-lymphoid Tissue
Tissue Digestion for Stromal Cell and Leukocyte Isolation
T-cell Response in the Lung Following Influenza Virus Infection. .
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