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RNA Vaccines Methods and Protocols
Title
RNA Vaccines [electronic resource] : Methods and Protocols / edited by Thomas Kramps, Knut Elbers.
ISBN
9781493964819
Publication
New York, NY : Springer New York : Imprint: Humana Press, 2017.
Physical Description
1 online resource (XI, 282 p.) 42 illus., 26 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume provides an overview of the field and practical hints for vaccinologists in academia and industry. Chapters provide protocols on self-replicating RNA vectors, non-replication mRNA vectors, adjuvantation and delivery, and preclinical and clinical development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Vaccines: Methods and Protocols aims to increased collaboration on RNA vaccines between basic and applied scientists in academia, government, and industry to develop future solutions for today’s challenges.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
August 31, 2018
Series
Methods in Molecular Biology, 1499
Contents
Introduction to RNA Vaccines
Self-replicating RNA
Self-replicating RNA Vaccine Delivery to Dendritic Cells
Plant Expression of Trans-encapsidated Viral Nanoparticle Vaccines with Animal RNA Replicons
RNActive® Technology: Generation and Testing of Stable and Immunogenic mRNA Vaccines
Nucleoside Modified mRNA Vaccines for Infectious Diseases
Generation and Evaluation of Prophylactic mRNA Vaccines against Allergy
Measuring the Adjuvant Activity of RNA Vaccines
Generation of Immunostimulating 130 nm Protamine-RNA Nanoparticles
Electroporation of mRNA as Universal Technology Platform to Transfect a Variety of Primary Cells with Antigens and Functional Proteins
Adjuvant-Enhanced mRNA Vaccines
Enhanced Delivery of DNA or RNA Vaccines by Electroporation
The European Regulatory Environment of RNA-Based Vaccines
Discovery and Subtyping of Neo-Epitope Specific T-cell Responses for Cancer Immunotherapy: Addressing the Mutanome
Considerations for Producing mRNA Vaccines for Clinical Trials
Nonclinical Safety Testing of RNA Vaccines
Immunotherapy of Uveal Melanoma: Vaccination Against Cancer.
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