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Extracellular RNA Methods and Protocols
Title
Extracellular RNA [electronic resource] : Methods and Protocols / edited by Tushar Patel.
ISBN
9781493976522
Publication
New York, NY : Springer New York : Imprint: Humana Press, 2018.
Physical Description
1 online resource (XI, 216 p.) 43 illus., 30 illus. in color.
Local Notes
Access is available to the Yale community.
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Access restricted by licensing agreement.
Summary
This volume provides a collection of methods and protocols detailing extracellular RNA and hopes to be a useful resource for those interested in this exciting and emerging field. Chapters are divided into four sections covering an overview of the field, approaches for the isolation of exRNA, protocols for detection and quantitation of exRNA, and approaches that can be useful for studies to evaluate potential therapeutic applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Extracellular RNA: Methods and Protocols aims to ensure successful results in the further study of this vital field. .
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Springer protocols (Series)
Other formats
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
August 31, 2018
Series
Methods in Molecular Biology, 1740
Contents
Extracellular RNAs – A New Awareness of Old Perspectives
Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles
Extracellular RNA Isolation from Cell Culture Supernatant
Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture
Isolation of Extracellular RNA from Serum/Plasma
Isolation of Extracellular RNA from Bile
Cushioned–Density Gradient Ultracentrifugation (C–DGUC): a Refined and High Performance Method for the Isolation, Characterization & Use of Exosomes
Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids
Enrichment of Extracellular Vesicle Subpopulations via Affinity Chromatography
Detection and Analysis of Non-vesicular Extracellular RNA
Isolation of Plasma Lipoproteins as a Source of Extracellular RNA
Droplet Digital PCR for Quantitation of Extracellular RNA
Preparation of Small RNA NGS Libraries from Biofluids
Multiplexed Detection and Quantitation of Extracellular Vesicle RNA Expression using NanoString
Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs
Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs. .
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