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Nanoscale Imaging Methods and Protocols
Title
Nanoscale Imaging [electronic resource] : Methods and Protocols / edited by Yuri L. Lyubchenko.
ISBN
9781493985913
Publication
New York, NY : Springer New York : Imprint: Humana Press, 2018.
Physical Description
1 online resource (XIX, 600 p.) 176 illus., 170 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume presents readers with the latest techniques to study nanoimaging and nanoprobing in application to a broad range of biological systems. The chapters in this book are divided into five parts, and cover topics such as imaging and probing of biomacromolecules including high-speed imaging and probing with AFM; probing chromatin structure with magnetic tweezers; and fluorescence correlation spectroscopy on genomic DNA in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and through, Nanoscale Imaging: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing and expanding field.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
August 31, 2018
Series
Methods in Molecular Biology, 1814
Contents
High Resolution Atomic Force Microscopy Imaging of Nucleic Acids
Two Ligand Binding Sites in Serotonin Transporter Revealed by Nanopharmacological Force Sensing
AFM-Based Single-Molecule Force-Spectroscopy of Proteins
High Resolution AFM-Based Force Spectroscopy
Polymer Nanoarray Approach for the Characterization of Biomolecular Interactions
Measuring Single-Molecule Twist and Torque in Multiplexed Magnetic Tweezers
AFM-Based Characterization of Electrical Properties of Materials
Supported Lipid Bilayers for Atomic Force Microscopy Studies
Quantifying Small Molecule Binding Interactions with DNA Nanostructures
Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy
High Resolution and High-Speed Atomic Force Microscope Imaging
High-Speed Atomic Force Microscopy of Individual Amyloidogenic Protein Assemblies
Direct Observation of Dynamic Movement of DNA Molecules in DNA Origami Imaged using High-Speed AFM
Assembly of Centromere Chromatin for Characterization By High-Speed Time-Lapse Atomic Force Microscopy
High-Speed Force Spectroscopy for Single Protein Unfolding
Probing RNA-Protein Interactions with Single-Molecule Pull-Down Assays
Preparing Frozen-Hydrated Protein-Nucleic Acid Assemblies for High-Resolution Cryo-EM Imaging
Probing Chromatin Structure with Magnetic Tweezers
Single Molecule and Ensemble Methods to Probe Initial Stages of RNP Granule Assembly
Correlative Atomic Force and Single Molecule Fluorescence Microscopy of Nucleoprotein Complexes
Sensing the Ultrastructure of Bacterial Surfaces and their Molecular Binding Forces using AFM
Nanoscale Visualization of Bacterial Microcompartments using Atomic Force Microscopy
Time-Resolved Imaging of Bacterial Surfaces using Atomic Force Microscopy
Probing Bacterial Adhesion at the Single-Molecule and Single-Cell Levels by AFM-Based Force Spectroscopy
Fluorescence Correlation Spectroscopy on Genomic DNA in Living Cells
Three-Dimensional Tracking of Quantum Dot-Conjugated Molecules in Living Cells
AFM Indentation Analysis of Cells to Study Cell Mechanics and Pericellular Coat
Imaging of Soft and Biological Samples using AFM Ringing Mode
Probing Single Virus Binding Sites on Living Mammalian Cells using AFM
Applications of Atomic Force Microscopy for Adhesion Force Measurements in Mechanotransduction
Methods for Atomic Force Microscopy of Biological and Living Specimens
Single Molecule Imaging in Live Embryos using Lattice Light-Sheet Microscopy
Silver Filler Pre-Embedding to Enhance Resolution and Contrast in Multidimensional SEM: A Nanoscale Imaging Study on Liver Tissue
Nanoscale Dynamics and Energetics of Proteins and Protein-Nucleic Acid Complexes in Classical Molecular Dynamics Simulations.
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