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RNA Detection Methods and Protocols
Title
RNA Detection [electronic resource] : Methods and Protocols / edited by Imre Gaspar.
ISBN
9781493972135
Publication
New York, NY : Springer New York : Imprint: Humana Press, 2018.
Physical Description
1 online resource (XIV, 492 p.) 94 illus., 78 illus. in color.
Local Notes
Access is available to the Yale community.
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Access restricted by licensing agreement.
Summary
This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.
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Springer protocols (Series)
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Format
Books / Online
Language
English
Added to Catalog
August 31, 2018
Series
Methods in Molecular Biology, 1649
Contents
The Secret Life of RNA: Lessons from Emerging Methodologies
Quantification of 2'-O-Me Residues in RNA using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)
Identifying the m6A Methylome by Affinity Purification and Sequencing
PARIS: Psoralen Analysis of RNA Interactions and Structures with High Throughput and Resolution
Axon-TRAP-RiboTag: Affinity-Purification of Translated mRNAs from Neuronal Axons in Mouse In Vivo
LCM-Seq: A Method for Spatial Transcriptomic Profiling using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing
Spatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas
Single mRNA Molecule Detection in Drosophila
Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryos
Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction
Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections
Hybridization Chain Reaction for Direct mRNA Detection without Nucleic Acid Purification
In Situ Detection of MicroRNA Expression with RNAscope Probes
Padlock Probes to Detect Single Nucleotide Polymorphisms
Quantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons
Optimizing Molecular Beacons for Intracellular Analysis of RNA
Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH
In Vivo Visualization and Function Probing of Transport mRNPs using Injected FIT Probes
Visualizing RNA in Live Bacterial Cells using Fluorophore- and Quencher-Binding Aptamers
Method for Imaging Live-Cell RNA using an RNA Aptamer and a Fluorescent Probe
RNA Live Imaging in the Model Microorganism Ustilago maydis
Real-Time Fluorescence Imaging of Single-Molecule Endogenous Non-Coding RNA in Living Cells
Live Imaging of mRNA Synthesis in Drosophila
Imaging Newly Transcribed RNA in Cells by Using a Clickable Azide-Modified UTP Analog
Detection of the First Round of Translation: The TRICK Assay
Imaging Translation Dynamics of Single mRNA Molecules in Live Cells
Systematic Detection of Poly(A)+ RNA-Interacting Proteins and Their Differential Binding
Isolation and Characterization of Endogenous RNPs from Brain Tissues
Individual Nucleotide Resolution UV Cross-Linking and Immunoprecipitation (iCLIP) to Determine Protein-RNA Interactions
RNA Tagging: Preparation of High-Throughput Sequencing Libraries
RAP-MS: A Method to Identify Proteins that Interact Directly with a Specific RNA Molecule in Cells.
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