Books+ Search Results
Current Laboratory Methods in Neuroscience Research
Title
Current Laboratory Methods in Neuroscience Research [electronic resource] / edited by Huangui Xiong, Howard E. Gendelman.
ISBN
9781461487944
Publication
New York, NY : Springer New York : Imprint: Springer, 2014.
Physical Description
1 online resource (XIII, 585 p.) 189 illus., 122 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
Current Laboratory Methods in Neuroscience Research is a research manual for both students and seasoned researchers. It focuses on commonly-used techniques employed in neuroscience research, presented in a simple, step-by-step manner for laboratory use. The manual also offers a “blueprint” for bench-to-bedside research designed to facilitate multidisciplinary neuroscience pursuits. Sections include coverage of neurohistological techniques, in vitro preparations, leukocyte isolation and application in neuroscience, standard laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, analysis of gene expression, and animal models.
Variant and related titles
Springer ENIN.
Other formats
Printed edition:
Printed edition:
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
April 11, 2019
Series
Springer Protocols Handbooks,
Contents
Section 1: Neurohistological techniques
1. Brain tissue preparation, sectioning and staining
2. Brain stereotactic injections
3. Tract tracing at light and electron microscopy
4. Stereological analysis of neurological tissues
Section 2: In vitro preparations
5. Preparation and use of rodent hippocampal brain slices
6. Single-Cell Neuronal Dissociation For Electrophysiological Studies
7. Isolation and Culture of Human Neurons, Microglia and Astrocytes
8. Isolation and Culture of Neural Stem/progenitor Cells
9. Isolation of mitochondria from brain tissue and cells
10. Isolation of synaptosomes from archived brain tissues
11. Xenopus oocytes isolation and microinjection
Section 3: Leukocyte isolation and application in neuroscience
12. Centrifugal elutriation for studies of neuroimmunity
13. Flow cytometry in neuroscience research
Section 4: Standard Laboratory Nucleic Acid and Protein Detections
14. Western Blotting Technique in Biomedical Research
15. Polymerase chain reaction (PCR) and Real-time RT PCR
Section 5: Nanomedicine
16. Nanoformulations
17. Neuronanomedicine
Section 6: Bioimaging
18. Confocal imaging of nerve cells
19. Magnetic resonance imaging (MRI)
20. X-Ray, Positron Emission, and Single Photon Emission Tomographic Bioimaging
21. Non-Invasive Neurophysiological Imaging with Magnetoencephalography
22. Multiphoton Brain Imaging
Section 7: Neuroelectrophysiology
23. Techniques for Extracellular Recordings
24. “Blind” patch-clamp recordings from rodent hippocampal slices
25. Whole-cell patch clamp recordings
Section 8: Immunohistochemistry and autoradiography
26. Analysis of Receptor Binding And Quantitative Autoradiography
27. Techniques in Immunohistochemistry and immunocytochemistry
Section 9: Analysis of Gene Expression
28. Protein identification by mass spectrometry: Proteomics
29. DNA Microarrays as a Tool for Neurosciences Research
30. Metabolomics
31. Bioinformatic Methods and Resources for Neuroscience Research
Section 10: Animal Models: Behavior and Pathology
32. Motor function in rodent models of neurodegenerative disorders
33. Humanized mice
34. Animal models for PD and ALS
35. Animal models for Alzheimer’s Disease
36. Animal Models: Behavior and Pathology.
Subjects
Also listed under
Bookmark As
Citation
