pt. I. Introduction
1. Hello BLAST
pt. II. Theory
2. Biological Sequences
3. Sequence Alignment
4. Sequence Similarity
pt. III. Practice
5. BLAST
6. Anatomy of a BLAST Report
7. A BLAST Statistics Tutorial
8. 20 Tips to Improve Your BLAST Searches
9. BLAST Protocols
pt. IV. Industrial-Strength BLAST
10. Installation and Command-Line Tutorial
11. BLAST Databases
12. Hardware and Software Optimizations
pt. V. BLAST Reference
13. NCBI-BLAST Reference
14. WU-BLAST Reference
pt. VI. Appendixes
A. NCBI Display Formats
B. Nucleotide Scoring Schemes
C. NCBI-BLAST Scoring Schemes
D. blast-imager.pl
E. blast2table.pl.