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Pollen and Pollen Tube Biology Methods and Protocols
Title
Pollen and Pollen Tube Biology [electronic resource] : Methods and Protocols / edited by Anja Geitmann.
ISBN
9781071606728
Edition
1st ed. 2020.
Publication
New York, NY : Springer US : Imprint: Humana, 2020.
Physical Description
1 online resource (XIII, 332 p.) 97 illus., 90 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume explores a collection of experimental techniques used to investigate different aspects of pollen development and function, including its role in reaching the ovule and delivering the two sperm cells. The techniques discussed range from basic methodology to cultivate pollen in vitro to the sophisticated experiments involving micromanipulation, Lab-on-Chip technology, or high-end imaging. The chapters in this book cover topics such as pollen grain counting using a cell counter; restricted pollination for tracing individual pollen tubes in a pistil; obtaining mutant pollen for phenotypic analysis and pollen tube dual-staining; analyzing intracellular gradients in pollen tubes; and measuring exocytosis rate in Arabidopsis pollen tube using corrected fluorescence recovery after photoconversion (cFRAPc) technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Pollen and Pollen Tube Biology: Methods and Protocols is a valuable resource for novice and expert scientists interested in learning more about the field of plant reproduction. .
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Springer protocols (Series)
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Printed edition:
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
June 19, 2020
Series
Methods in Molecular Biology, 2160
Contents
Pollen Grain Counting Using a Cell Counter
A Toolkit for teasing Apart the Early Stages of Pollen-Stigma Interactions in Arabidopsis thaliana
Decapitation Crosses to Test Pollen Fertility Mutations for Defects in Stigma-Style Penetration
Isolation of the Pistil-Stimulated Pollen Tube Secretome
Restricted Pollination for Tracing Individual Pollen Tubes in a Pistil
Semi-In Vivo Assay for Pollen Tube Attraction
Isolation and Cloning of Suppressor Mutants with Improved Pollen Fertility
Workflow to Characterize Mutants with Reproductive Defects
Internally-Controlled Methods to Quantify Pollen Tube Growth and Penetration Defects in Arabidopsis thaliana
Arabidopsis thaliana Pollen Tube Culture for Multi-Omics Studies
Large Scale Analysis of Pollen Viability and Oxidative Level using H2DCFDA-Staining Coupled with Flow Cytometry
Obtaining Mutant Pollen for Phenotypic Analysis and Pollen Tube Dual-Staining
Galvanotropic Chamber for Controlled Reorientation of Pollen Tube Growth and Simultaneous Confocal Imaging of Intracellular Dynamics
Analyzing Intracellular Gradients in Pollen Tubes
Silicone Chambers for Pollen Tube Imaging in Microstructures In Vitro Environments
Analyzing the Impact of Protein Overexpression on Ca2+ Dynamics and Development in Tobacco Pollen Tubes
Imaging and Analysis of the Content of Callose, Pectin, and Cellulose in the Cell Wall of Arabidopsis Pollen Tubes Grown In Vitro
Characterization of Growth Behavior and the Resulting Forces Applied by Pollen Tubes in a 3D Matrix
Flow Chamber Assay to Image the Response of FRET-Based Nanosensors in Pollen Tubes to Changes in Medium Composition
Quantification of Mechanical Forces and Physiological Processes Involved in Pollen Tube Growth Using Microfluidics and Microrobotics
Measuring Exocytosis Rate in Arabidopsis Pollen Tube Using Corrected Fluorescence Recovery after Photoconversion (cFRAPc) Technique
Testing Pollen Tube Proteins for In Vivo Binding to Phosphatidic Acid by n0Butanol Treatment and Confocal Microscopy.
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