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Dynamin Superfamily GTPases Methods and Protocols
Title
Dynamin Superfamily GTPases [electronic resource] : Methods and Protocols / edited by Rajesh Ramachandran.
ISBN
9781071606766
Edition
1st ed. 2020.
Publication
New York, NY : Springer US : Imprint: Humana, 2020.
Physical Description
1 online resource (X, 221 p.) 53 illus., 33 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume provides methods and protocols on dynamin superfamily GTPases. Chapters are divided into three parts; detailing expression, purification, initial biochemical steps, undescribed analytical techniques, and biophysical and cellular characterization of DSPs in membrane remodeling, fission and fusion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Dynamin Superfamily GTPases: Methods and Protocols to ensure successful results in the further study of this vital field.
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Springer protocols (Series)
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Format
Books / Online
Language
English
Added to Catalog
June 19, 2020
Series
Methods in Molecular Biology, 2159
Contents
Isolation and Analysis of Mitochondrial Fission Enzyme DNM1 from Saccharomyces cerevisiae
Purification of the Dynamin-related Protein Vps1 using Mammalian and Bacterial Expression Systems
A Single Common Protocol for the Expression and Purification of Soluble Mammalian DSPs from Escherichia coli
Affinity Purification and Functional Characterization of Dynamin-related Protein 1
Purification and Characterization of MxB
Purification of Farnesylated hGBP1 and Characterization of Its Polymerization and Membrane Binding
Microscale Thermophoresis (MST) as a Tool for Measuring Dynamin Superfamily Protein (DSP)-lipid Interactions
Nucleotide-dependent Dimerization and Conformational Switching of Atlastin
Analysis of Mitochondrial Membrane Fusion GTPase OPA1 Expressed by the Silkworm Expression System
Cell-free Analysis of Mitochondrial Fusion by Fluorescence Microscopy
Electrophysiological Methods For Detection Of Membrane Leakage and Hemifission by Dynamin 1
Integrating Optical and Electrochemical Approaches to Assess the Actions of Dynamin at the Fusion Pore
Cellular Assays for Measuring Dynamin Activity in Muscle Cells
Measuring Drp1 Activity in Mitochondrial Fission in vivo
Quantifying Drp1-mediated Mitochondrial Fission by Immunostaining in Fixed Cells
Imaging Dynamin-related Protein 1 (Drp1)-mediated Mitochondrial Fission in Living Cells. .
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