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Leptospira spp Methods and Protocols
Title
Leptospira spp [electronic resource] : Methods and Protocols / edited by Nobuo Koizumi, Mathieu Picardeau.
ISBN
9781071604595
Edition
1st ed. 2020.
Publication
New York, NY : Springer US : Imprint: Humana, 2020.
Physical Description
1 online resource (X, 291 p.) 66 illus., 56 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume looks at all aspects of manipulation of Leptospira spp. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from both clinical and environmental samples; using methods like whole genome sequencing and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry to identify bacterial species; tools for gene inactivation and in vitro and in vivo assays to study the pathogenesis of leptospirosis; and the use of hamsters to evaluate leptospiral virulence and vaccine candidates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Leptospira spp.: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field and these fascinating organisms. .
Variant and related titles
Springer protocols (Series)
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Printed edition:
Format
Books / Online
Language
English
Added to Catalog
December 21, 2020
Series
Methods in Molecular Biology, 2134
Contents
Isolation and Culture of Leptospira from Clinical and Environmental Samples
Core Genome Multi-Locus Sequence Typing Analyses of Leptospira spp. using the Bacterial Isolate Genome Sequence Database
Use of MALDI-ToF Mass Spectrometry for Identification of Leptospira
Cell Enumeration of Leptospira by Flow Cytometry
The Single-Step Method of RNA Purification Applied to Leptospira
Purification of LPS from Leptospira
Isolation, Purification, and Characterization of Leptophages
Creating a Library of Random Transposon Mutants in Leptospira
Transposon Sequencing in Leptospira interrogans
Specific Gene Silencing in Leptospira biflexa by RNA-Guided Catalytically inactive Cas9 (dCas9)
Leptospira spp. Toolbox for Chemotaxis Assay
In Situ Structural Analysis of Leptospira spp. by Electron Cryotomography
Measurement of the Cell-Body Rotation of Leptospira
In Vivo Imaging of Bioluminescent Leptospires
Cell Monolayer Translocation Assay
Cell Adhesion Assay to Study Leptospiral Proteins: An Approach to Investigate Host-Pathogen Interaction
Complement Resistance Assays
Evaluation of Intracellular Trafficking in Macrophages
Biofilm Formation and Quantification using the 96-Microtiter Plate
Survival Tests for Leptospira spp.
Cultivation of Leptospira interrogans within Rat Peritoneal Dialysis Membrane Chambers
Use of Golden Syrian Hamster as an Animal Model to Study Leptospirosis-Associated Immune Responses
Evaluation of Vaccine Candidates against Leptospirosis using Golden Syrian Hamsters
Leptospira and Leptospirosis
Laboratory Diagnosis of Leptospirosis.
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