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Shotgun Proteomics Methods and Protocols
Title
Shotgun Proteomics [electronic resource] : Methods and Protocols / edited by Mónica Carrera, Jesús Mateos.
ISBN
9781071611784
Edition
1st ed. 2021.
Publication
New York, NY : Springer US : Imprint: Humana, 2021.
Physical Description
1 online resource (XVII, 324 p.) 59 illus., 49 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This edition details a collection of specific shotgun proteomics-based laboratory techniques and applications developed in leading laboratories and proteomics units worldwide. Chapters cover a broad range of topics covering, shotgun proteomics of extracellular vesicles and subcellular structures, shotgun proteomics in non-model organisms, clinical proteomics, food proteomics, analysis of post-translational modifications and protein complexes, and data processing and storage. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Shotgun Proteomics: Methods and Protocols aims to be an up-to-date guide for researchers seeking to understand the proteome of any given biological sample.
Variant and related titles
Springer protocols (Series)
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Format
Books / Online
Language
English
Added to Catalog
April 12, 2021
Series
Methods in Molecular Biology, 2259
Contents
Mesenchymal Stem Cell - Derived Extracellular Vesicles Isolation and their Protein Cargo Characterisation
Clinical Proteomics for the Analysis of Circulating Extracellular Vesicles
Utilization of Laser Capture Microdissection Coupled to Mass Spectrometry to Uncover the Proteome of Cellular Protrusions
Isolation of Apoplastic Fluid From Woody Plants Leaves: Grapevine and Coffee as a Case Study
Shotgun Proteomics for L3 and L4 Anisakis simplex Development Stages
A Primer and Guidelines for Shotgun Proteomic Analysis in Non-Model Organisms
SWATH-MS Protocols in Human Diseases
Serum Proteomic Profiling in Rheumatoid Arthritis by Antibody Suspension Bead Arrays
Human Blood Plasma Investigation Employing 2d Uplc-Udmse Data-Independent Acquisition Proteomics
Metaproteomics Analysis of Host-Microbiota Interfaces
Mass Spectrometry-Based Analysis of Mycobacterial Single-Colony Proteome
Proteogenomic Approach for Mycobacterium Tuberculosis Investigation
Shotgun Proteomics for Food Microorganism Detection
Shotgun Proteomics and Protein-Based Bioinformatics for the Characterization of Food-Derived Bioactive Peptides
FTSC-labeling Coupled with 2DE-LC-MS/MS Analysis of Complex Protein Mixtures for Identification and Relative Quantification of Tissue Carbonylome
Mass Spectrometry-Based Proteomics for Analysis of Hydrophilic Phosphopeptides
Rapid Shotgun Phosphoproteomics Analysis
System-wide Profiling Of Protein-Complexes Via Size Exclusion Chromatography-Mass Spectrometry (SEC-MS)
Qualitative and Quantitative Shotgun Proteomics Data Analysis from Data-Dependent Acquisition Mass Spectrometry
The jPOST Repository as a Public Data Repository for Shotgun Proteomics.
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