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Ras Activity and Signaling Methods and Protocols
Title
Ras Activity and Signaling [electronic resource] : Methods and Protocols / edited by Ignacio Rubio, Ian Prior.
ISBN
9781071611906
Edition
1st ed. 2021.
Publication
New York, NY : Springer US : Imprint: Humana, 2021.
Physical Description
1 online resource (XII, 439 p.) 86 illus., 57 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume compiles new experimental approaches and concepts focusing mostly, but not solely, on ways to manipulate and regulate Ras activity and its downstream signaling output. Chapters detail standard methodologies, biochemical methods, Ras processing trafficking and localization, Ras signaling and inhibition, and in vivo models for studying Ras function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Ras Activity and Signaling: Methods and Protocols aims to provide support and guidance to lab workers in their work on Ras GTPases and in the design of new projects requiring novel methodologies.
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Springer protocols (Series)
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Format
Books / Online
Language
English
Added to Catalog
June 03, 2021
Series
Methods in Molecular Biology, 2262
Contents
Ras Variant Biology and Contributions to Human Disease
Regulation of the Small GTPase Ras and its Relevance to Human Disease
Precise Characterization of KRAS4B Proteoforms by Combining Immunoprecipitation with Top-Down Mass Spectrometry
Absolute Quantitation of GTPase Protein Abundance
Validation of Isoform- and Mutation-specific RAS Antibodies
Production and Membrane Binding of N-terminally Acetylated, C-terminally Farnesylated and Carboxymethylated KRAS4b
Active GTPase Pulldown Protocol
Methods to Monitor Ras Activation State
NMR Detection Methods for Profiling RAS Nucleotide Cycling
Ras Diffusion and Interactions with the Plasma Membrane Measured by FRAP Variations
Understanding Ras Spatial Cycles through Reaction-Diffusion Simulations
Super-Resolution Imaging and Spatial Analysis of RAS on Intact Plasma Membrane Sheets
FLIM-FRET Analysis of Ras Nanoclustering and Membrane-Anchorage
Assessment of Plasma Membrane Fatty Acid Composition and Fluidity Using Imaging Flow Cytometry
Spatiotemporal Imaging of Small GTPase Activity using Conformational Sensors for GTPase Activity (COSGA)
Using BioID to Characterize the RAS Interactome
Probing RAS function with Monobodies
Detection of Endogenous RASSF1A Interacting Proteins
Mathematical Modeling to Study KRAS Mutant-Specific Responses to Pathway Inhibition
A Facile Method to Engineer Mutant Kras Alleles in an Isogenic Cell Background
RASless MEFs as a Tool to Study RAS-dependent and -independent Functions
Generation of Patient-derived Colorectal Cancer Organoids for RAS Studies
Ras-GEF Mouse Models for the Analysis of Ras Biology and Signaling
Studying Metabolic Abnormalities in the Costello Syndrome HRAS G12V Mouse Model: Isolation of Mouse Embryonic Fibroblasts and Their In Vitro Adipocyte Differentiation
Dissecting Oncogenic RAS Signalling in Melanoma Development in Genetically Engineered Zebrafish Models
Ras, Ral, and Rap1 in C. elegans. .
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