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PDZ Mediated Interactions Methods and Protocols
Title
PDZ Mediated Interactions [electronic resource] : Methods and Protocols / edited by Jean-Paul Borg.
ISBN
9781071611661
Edition
1st ed. 2021.
Publication
New York, NY : Springer US : Imprint: Humana, 2021.
Physical Description
1 online resource (XI, 292 p.) 100 illus., 50 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume provides readers with a comprehensive look at the latest techniques used to identify and characterize PDZ-mediated interactions. Chapters cover topics such as promiscuity, multimodularity, regulation, and viral recognition by PDZ domains. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, PDZ Mediated Interactions: Methods and Protocols is a valuable resource for all researchers interested in learning more about this developing field. .
Variant and related titles
Springer protocols (Series)
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Format
Books / Online
Language
English
Added to Catalog
June 03, 2021
Series
Methods in Molecular Biology, 2256
Contents
Identification of PDZ Interactions by Yeast Two-Hybrid
Identification of PDZ Interactions by Affinity Purification and Mass Spectrometry Analysis
Identification of PDZ Interactions by Proteomic Peptide Phage Display
A Computational Protocol to Analyze PDZ/PBM Affinity Data Obtained by High-Throughput Holdup Assay
Study of PDZ-Peptide and PDZ-Lipid Interactions by Surface Plasmon Resonance/BIAcore
PDZ Sample Quality Assessment by Biochemical and Biophysical Characterizations
Crystallographic Studies of PDZ Domain-Peptide Interactions of the Scribble Polarity Module
A Fluorescence-Based Assay to Determine PDZ-Ligand Binding Thermodynamics
Unveiling the Folding Mechanism of PDZ Domains
Development of Peptide-Based PDZ Domain Inhibitors
Dynamic Control of Signaling by Phosphorylation of PDZ Binding Motifs
Chemical Synthesis of PDZ Domains
Viral PDZ Binding Motifs Influence Cell Behavior through the Interaction with Cellular Proteins Containing PDZ Domains
Computational Design of PDZ:peptide Binding
Mechanoregulation of PDZ Proteins, An Emerging Function
Rational Design of PDZ Domain Inhibitors: Discovery of Small Organic Compounds Targeting PDZ Domains.
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