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Neuronal Cell Culture Methods and Protocols
Title
Neuronal Cell Culture [electronic resource] : Methods and Protocols / edited by Shohreh Amini, Martyn K. White.
ISBN
9781071614372
Edition
2nd ed. 2021.
Publication
New York, NY : Springer US : Imprint: Humana, 2021.
Physical Description
1 online resource (XII, 205 p.) 50 illus., 42 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies. .
Variant and related titles
Springer protocols (Series)
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Printed edition:
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
June 03, 2021
Series
Methods in Molecular Biology, 2311
Contents
General Overview of Neuronal Cell Culture
Considerations for the use of SH-SY5Y Neuroblastoma Cells in Neurobiology
Cultured cell line models of neuronal differentiation: NT2, PC12, SK-N-MC
Murine teratocarcinoma-derived neuronal cultures
Isolation and propagation of primary human and rodent embryonic neural progenitor cells and cortical neurons
Mouse Enteric Neuronal Cell Culture
Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications
Slice culture modeling of CNS viral infection
Neurospheres and Glial cell cultures: immunocytochemistry for cell phenotyping
Transfection of Neuronal Cultures
Lentiviral Transduction of Neuronal Cells
Compartmentalized Neuronal Cultures
Quantitative assessment of neurite outgrowth over growth promoting or inhibitory substrats
Isolation and Culture of Dorsal Root Ganglia (DRG) from Rodents
Isolation of Primary Human and Rodent Brain Microvascular Endothelial Cells: Culturing, Characterization, and High-Efficiency Transfection
Fetal Brain Injury Models of Fetal Alcohol Syndrome: Examination of Neuronal Morphologic Condition Using Sholl Assay. .
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