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Mitochondrial Medicine Volume 2: Assessing Mitochondria
Title
Mitochondrial Medicine [electronic resource] : Volume 2: Assessing Mitochondria / edited by Volkmar Weissig, Marvin Edeas.
ISBN
9781071612668
Edition
2nd ed. 2021.
Publication
New York, NY : Springer US : Imprint: Springer, 2021.
Physical Description
1 online resource (XVI, 459 p.) 112 illus., 75 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional' mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D optical cryo-imaging method, mitochondrial ATP and ROS production, protocol for untargeted metabolomic analysis, and methods for analysis of nitrotyrosine-containing proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Mitochondrial Medicine, Second Edition, Volume 2: Assessing Mitochondria aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine.
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Format
Books / Online
Language
English
Added to Catalog
June 03, 2021
Series
Methods in Molecular Biology, 2276
Contents
Mitochondrial Dysfunction in Mitochondrial Medicine: Current Limitations, Pitfalls, and Tomorrow
Preparation of 'Functional' Mitochondria - A Challenging Business
Isolation and Quality Control of Functional Mitochondria
Purification of Functional Platelet Mitochondria Using a Discontinuous Percoll Gradient
Mechanical Permeabilization as a New Method for Assessment of Mitochondrial Function in Insect Tissues
Analysis of Mitochondrial Retrograde Signaling In Yeast Model Systems
Native Gel Electrophoresis and Immunoblotting to Analyze Electron Transport Chain Complexes
Measuring Mitochondrial Hydrogen Peroxide Levels and Glutathione Redox Equilibrium in Drosophila Neuron Subtypes Using Redox-Sensitive Fluorophores and 3D Imaging
Assessment of Mitochondrial Cell Metabolism By Respiratory Chain Electron Flow Assays
Whole-Cell and Mitochondrial dNTP Pool Quantification from Cells and Tissues
Single Particle-Tracking Method in Fluorescence Microscopy to Monitor Bioenergetic Responses Of Individual Mitochondria
Investigation of Mitochondrial ADP-ribosylation via Immunofluorescence
Assessment of Mitochondrial Ca2+ Uptake
Assessment of Mitochondrial Membrane Potential and NADH Redox State in Acute Brain Slices
Evaluation of Mitochondria Content and Function in Live Cells by Multi-color Flow Cytometric Analysis
Analysis of Mitochondrial Dysfunction during Cell Death
Modified Blue-Native Gel Approach for Analysis of Respiratory Super-Complexes
Patch-Clamp Recording of the Activity of Ion Channels in the Inner Mitochondrial Membrane
Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway
3D Optical Cryo-Imaging Method: A Novel Approach To Quantify Renal Mitochondrial Bioenergetics Dysfunction
Simultaneous Quantification of Mitochondrial ATP and ROS Production Using ATP Energy Clamp Methodology
High-Throughput Image Analysis of Lipid-Droplet-Bound Mitochondria
Cell Energy Budget Platform for Multi-Parametric Assessment of Cell and Tissue Metabolism
Fluorescence-based Assay for Measuring OMA1 Activity
Studying Mitochondrial Network Formation by in vivo and in vitro Reconstitution Assay
Extraction of Functional Mitochondria Based on the Membrane Stiffness
A Protocol for Untargeted Metabolomic Analysis: From Sample Preparation to Data Processing
A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled With Mass Spectrometry
In vivo Visualization And Quantification of Mitochondrial Morphology in C. elegans
Assessing Impact of Platinum Complexes on Mitochondrial Functions
In-silico Modeling of the Mitochondrial Pumping Complexes with Markov State Models
Monitoring the Mitochondrial Presequence Import Pathway in Living Mammalian Cells with a New Molecular Biosensor.
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