Cytochrome P450: In Vitro Methods and Protocols
Characteristics of Major Drug Metabolizing Cytochrome P450 Enzymes
Quantitative Determination of Cytochrome P450 Using LC/MS/MS
Spectral Quantitation of Total Cytochrome P450 in Microsomes Using a Single-Beam Spectrophotometer
Cytochrome P450 Enzyme Kinetics: Km and Vmax Determinations Using Metabolite Formation and Substrate Depletion Methods
Cytochrome P450 Inhibition Assay Using Human Liver Microsomes
Evaluation of Time-Dependent Cytochrome P450 Inhibition Using the Area Under the Curve Shift Method
Assessing Cytochrome P450 Time Dependent Inhibition (IC50 Shift Assay) Using Both Diluted and Non-Diluted Methods
Cytochrome P450 Gene Regulation: Reporter Assays to Assess Pregnane X Receptor (PXR, NR1I2) Activation
Cytochrome P450 Gene Regulation: Reporter Assays to Assess Aryl Hydrocarbon Receptor (HLHE76, AhR) Activation and Antagonism
Cytochrome P450 Gene Regulation: Reporter Assays to Assess Direct and Indirect Activation of Constitutive Androstane Receptor (CAR, NR1I3)
Determination of In Vitro Cytochrome P450 Induction Potential Using Cryopreserved Human Hepatocytes
Assessing Cytochrome P450 3A (CYP3A) Induction and Suppression Using Primary Human Hepatocyte Spheroids
Cytochrome P450-Mediated Metabolic Stability Assay in Liver Microsomes
Assessment of Cytochrome P450 Metabolic Clearance Using Hepatocyte Suspension
Cytochrome P450 Knock-Out Assay with Non-Selective Inhibitors
Differentiation of Cytochrome P450-Mediated from Non-CYP-Mediated Metabolism: Aldehyde Oxidase and Xanthine Oxidoreductase
In Vitro Reaction Phenotyping of Cytochrome P450 Enzymes
Cytochrome P450-Mediated Drug Bioactivation Assay: An Untargeted High Resolution Accurate Mass LC/MS Assay
Simultaneously Assessing Concentration Changes in 20 Biochemical Pathways as a Result of Drug Dosing and Cytochrome P450 and Non-Cytochrome P450-Mediated Metabolism: An Untargeted Metabolomics GC/MS Assay
Simultaneously Assessing Concentration Changes in 17 Biochemical Pathways as a Result of Drug Dosing and Cytochrome P450 and Non-Cytochrome P450-Mediated Metabolism: A Quasi-Untargeted Metabolomics LC/MS Assay
Assessing Amino Acid Concentration Changes as a Result of Drug Dosing and Cytochrome P450 and Non-Cytochrome P450-Mediated Metabolism.