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Receptor and Ion Channel Detection in the Brain
Title
Receptor and Ion Channel Detection in the Brain [electronic resource] / edited by Rafael Lujan, Francisco Ciruela.
ISBN
9781071615225
Edition
2nd ed. 2021.
Publication
New York, NY : Springer US : Imprint: Humana, 2021.
Physical Description
1 online resource (XIX, 554 p.) 109 illus., 80 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This second edition volume expands on the previous edition with new information on the latest developments in the field, and the most up-to-date representative methodological information on molecular neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters in this book are organized into three parts. Part One discusses molecular immunological and pharmacological aspects related to the detection of receptors and ion channels in the brain. Part Two explores the neuroanatomical techniques devoted to localizing receptors and ion channels within the central nervous system. Part Three looks at functional approaches devoted to ascertaining receptors and ion channels functionality in the brain. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Receptor and Ion Channels Detection in the Brain, Second Edition is a valuable resource that contains detailed molecular, cellular, neuroanatomical, and electrophysiological methods that will help researchers study neurotransmitter receptor and ion channel in any region of neuron of the brain.
Variant and related titles
Springer protocols (Series)
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Printed edition:
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Format
Books / Online
Language
English
Added to Catalog
September 22, 2021
Series
Neuromethods, 169
Contents
Production of High-Quality Antibodies for the Study of Receptors and Ion Channels
Co-Immunoprecipitation from Brain
Sub-Synaptic Membrane Fractionation
Quantitative Analysis of Cell Surface Expressed, Intracellular, and Internalized Neurotransmitter Receptor Populations in Brain Slices Using Biotinylation
Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cells
Radioligand Binding Detection of Receptors in Brain Membranes
Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain
Time Resolved-Fluorescence Resonance Energy Transfer using Fluorescent Ligands to Study Native G Protein-Coupled Receptor Heteromerization in Brain
Study of GPCR Homo and Heteroreceptor Complexes in Specific Neuronal Cell Populations using the In Situ Proximity Ligation Assay
Amplified Luminescent Proximity Homogeneous Assay (Alpha)-Based Technique to Detect GPCR Oligomers in Human Post-Mortem Brain
Fluorescent In Situ Hybridization for Sensitive and Specific Labeling
Autoradiographic Visualization of G Protein-Coupled Receptors in Brain
Localization of Neurotransmitter Receptor and Ion Channel Proteins in Unfixed Brains using In Situ Immunoblotting
Immunohistochemistry for Ion Channels and their Interacting Molecules: Tips for Improving Antibody Accessibility
Localization of GFP-Tagged Proteins at the Electron Microscope
Tyramide Signal Amplificación for Immunoelectron Microscopy
Pre-Embedding Methods for the Localization of Receptors and Ion Channels
Post-Embedding Immunohistochemistry in the Localization of Receptors and Ion Channels
High-Resolution Localization and Quantitation of Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL)
Pre-Embedding Immunostaining of Brain Tissue and Three-Dimensional Imaging with FIB-SEM
Application of Virus Vectors for Anterograde Tract-Tracing and Single-Neuron Labeling Studies
Efficient Labeling of Neurons and Identification of Postsynaptic Sites Using Adeno-Associated Virus Vector
Analysis of Synaptic Connections at the Electron Microscopic Level using Sindbis Virus Vectors
Morphological and Neurochemical Characterization of Electrophysiologically Identified Cells
Using Electrophysiology to Study Synaptic and Extrasynaptic Ionotropic Receptors in Hippocampal Neurons
Biophysical Methods to Analyze Direct G-Protein Regulation of Neuronal Voltage-Gated Calcium Channels
Electrophysiological Recordings in Behaving Animals
Voltammetry in Behaving Animals
In Vivo Brain Microdialysis of Monoamines
Optical Control of Brain Receptors using Photoactive Drug in Behaving Animals
Dynamic Recording of Membrane Potential from Hippocampal Neurons by Using a Fluorescence Resonance Energy Transfer-Based Voltage Biosensor
Monitoring GPCR-Mediated cAMP Accumulation in Rat Striatal Synaptosomes
GPCR-Mediated MAPK/ERK Cascade Activation in Mouse Striatal Slices.
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