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Long Non-Coding RNAs Methods and Protocols
Title
Long Non-Coding RNAs [electronic resource] : Methods and Protocols / edited by Lin Zhang, Xiaowen Hu.
ISBN
9781071616970
Edition
2nd ed. 2021.
Publication
New York, NY : Springer US : Imprint: Humana, 2021.
Physical Description
1 online resource (XIII, 308 p.) 48 illus., 41 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This second edition provides a broad spectrum of methods used in long non-coding RNAs (lncRNA) research, ranging from computational annotation of lncRNA genes to molecular and cellular analyses of the function of individual lncRNA. Chapters guide readers through studies used to circular RNAs, RNA splicing, and findings on lncRNA in human diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Long Non-Coding RNAs: Methods and Protocols,Second Edition aims to ensure successful results in the further study of this vital field.
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Springer protocols (Series)
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Format
Books / Online
Language
English
Added to Catalog
September 22, 2021
Series
Methods in Molecular Biology, 2372
Contents
LncRNA pulldown Combined with Mass Spectrometry to Identify the Novel lncRNA-associated Proteins
Cross-Linking Immunoprecipitation and qPCR (CLIP-qPCR) Analysis to Map Interactions of Long Noncoding RNAs with Canonical and Non-canonical RNA-binding Proteins
Characterization of Long Non-coding RNA Associated Proteins by RNA-immunoprecipitation
Isolation of Protein Complexes Associated with Long Non-Coding RNAs
Detection of Long Noncoding RNA Expression by Real-time PCR
Profiling Long Non-coding RNA expression using Custom-Designed Microarray
Long Non-coding RNA Expression Profiling using Arraystar LncRNA Microarrays
Nuclear RNA Isolation and Sequencing
Capturing Endogenous Long Noncoding RNAs and Their Binding Proteins Using Chromatin Isolation by RNA Purification
Purification and Structural Characterization of the Long Noncoding RNAs
Simultaneous RNA-DNA FISH
Highly Resolved Detection of Long Non-coding RNAs In Situ
Detection of Long Non-coding RNA Expression by Non-radioactive Northern Blots
Assessment of in vivo siRNA Delivery in Cancer Mouse Models
Genetic Editing of Long Non-Coding RNA Using CRISPR/Cas9 Technology
Characterization of Circular RNAs
Identification of circRNA-Interacting Proteins by Affinity Pulldown
Exploration to Circular RNA Interactomes by RNA Pull-Down Method
Methods for Characterization of Alternative RNA Splicing
Computational analysis of lncRNA from cDNA sequences
Characterizing miRNA-lncRNA interplay
Illuminating lncRNA Function through Target Prediction
Long Noncoding RNAs: An Overview.
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