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Development of Mechanically Complex DNA Nanodevices and a Diverse DNA Origami Toolkit for CryoEM Imaging
Author
Title
Development of Mechanically Complex DNA Nanodevices and a Diverse DNA Origami Toolkit for CryoEM Imaging / John T Powell.
ISBN
9798534651577
Publication
[New Haven, Connecticut] : Yale University, 2021.
Physical Description
1 online resource (118 pages)
Notes
Advisors: Lin, Chenxiang Committee members: Matthew Simon; Yongli Zhang; Charles Sindelar.
Description based on Dissertations Abstracts International, Volume: 83-02, Section: B.
Access and use
Proquest dissertation: Access is restricted by licensing agreement.
EliScholar dissertation: Access is available to the Yale community
Summary
Mechanically interlocked supramolecular assemblies are appealing building blocks for creating functional nanodevices. Here we describe the multi-step assembly of massive DNA origami rotaxanes that are capable of programmable structural switching. We validated the topology and structural integrity of these rotaxanes by analyzing the intermediate and final products of various assembly routes using electrophoresis and electron microscopy. Additionally, we explore the use of various other DNA origami nanodevices as support structures for improved cryoEM imaging of proteins of interest. Namely, we demonstrate progress toward several DNA nanodevice-based solutions to artifacts stemming from air-water interface interactions and other freezing effects.
Variant and related titles
Proquest dissertation Dissertations & Theses @ Yale University.
Format
Books / Online
Language
English
Added to Catalog
December 16, 2021
Thesis note
Ph.D. Yale University 2021.
Subjects
Genre/Form
Academic theses.
Also listed under
Yale University. Department of Cell Biology, degree granting institution.
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