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Plant Virology Methods and Protocols
Title
Plant Virology [electronic resource] : Methods and Protocols / edited by Aiming Wang, Yi Li.
ISBN
9781071618356
Edition
1st ed. 2022.
Publication
New York, NY : Springer US : Imprint: Humana, 2022.
Physical Description
1 online resource (XIV, 320 p.) 50 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction assays, purification and transfection of protoplasts, protocols for gene silencing, and transmission electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Plant Virology: Methods and Protocols is a valuable resource for plant pathologists, microbiologists, virologists, graduate students, and teachers who are interested in learning more about the developments in plant virology research. .
Variant and related titles
Springer protocols (Series)
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Format
Books / Online
Language
English
Added to Catalog
February 21, 2022
Series
Methods in Molecular Biology, 2400
Contents
In Vivo Detection of Double-Stranded RNA by dRBFC Assay
Developing Rice Mutants using CRISPR/Cas9-Based Genome Editing Technology
Microscale Thermophoresis Assay: A Powerful Method to Quantify Protein-Nucleic Acid and Protein-Protein Interactions
Transient Expression-Mediated Gene Silencing in Plants and Suppression of Gene Silencing with Viral Suppressors
Isolation and Transfection of Plant Mesophyll Protoplasts for Virology Research
Split-Luciferase Complementation for Analysis of Virus-Host Protein Interactions
Monitoring Virus Intercellular Movement from Primary Infected Cells to Neighboring Cells in Plants
Simultaneous Determination and Subcellular Localization of Protein-Protein Interactions in Plant Cells using Bimolecular Fluorescence Complementation Assay
A Powerful Method for Studying Protein-Protein Interactions in Plants: Co-immunoprecipitation (Co-IP) Assay
Membrane and Nuclear Yeast Two-Hybrid Systems
Tag-Based Pull-Down Assay
Purification of Plasmodesmata-Enriched Fraction for Proteomic Analyses
Purification and Proteomics Analysis of Phloem Tissues from Virus-Infected Plants
Rapid and Specific Purification of Argonaute-Small RNA Complexes from Rice for Slicer Activity
Silencing Specific Genes in Plants Using Virus-Induced Gene Silencing (VIGS) Vectors
A Versatile Expression Platform in Insects and Cereals Based on a Cytorhabdovirus
Production of Virus-Free Chrysanthemum morifolium Ramat by Tissue Culture Techniques
Long-Term Preservation of Plant Viruses in Cryopreserved Shoot Tips
Establishment of White-Backed Planthopper Cell Lines
Biolistic Inoculation of Fruit Tress with Full-Length Infectious cDNA Clones of RNA Viruses
Purification of Total RNAs and Small RNAs from Fruit Tree Leaf Tissues
Diagnosis of Viral Diseases Using Deep Sequencing and Metagenomics Analyses
Immunocapture-Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay for Detection of Plant RNA Viruses
Detection of Tomato spotted wilt virus (TSWV) Infection in Plants using DAS-ELISA and Dot-ELISA
Tube Capture (TC) RT-PCR and Multiplex RT-PCR for Diagnosis and Characterization of Viruses in Fruit Trees
Detection of Cucumber green mottle mosaic virus (CGMMV) in Cucurbitaceous Crop Seeds by RT-PCR
RNA In Situ Hybridization of Detecting Cucumber Mosaic Virus in Shoots of Nicotiana benthamiana Plants
Transmission Electron Microscopic Methods for Plant Virology.
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