Books+ Search Results
Yeast Surface Display
Title
Yeast Surface Display [electronic resource] / edited by Michael W. Traxlmayr.
ISBN
9781071622858
Edition
1st ed. 2022.
Publication
New York, NY : Springer US : Imprint: Humana, 2022.
Physical Description
1 online resource (XV, 646 p.) 109 illus., 96 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Surface Display serves as a comprehensive resource that enables the implementation of this powerful and versatile technique in virtually any molecular biology laboratory, even in the absence of any prior yeast surface display experience.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Printed edition:
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
June 21, 2022
Series
Methods in Molecular Biology, 2491
Contents
Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System
Yeast Surface Display for Protein Engineering: Library Generation, Screening, and Affinity Maturation
Site-Wise Diversification of Combinatorial Libraries Using Insights from Structure-Guided Stability Calculations
Ancestral Sequence Reconstruction and Alternate Amino Acid States Guide Protein Library Design for Directed Evolution
Machine Learning-Driven Protein Library Design: A Path Toward Smarter Libraries
Kinetic Competition Screening of Yeast-Displayed Libraries for the Generation of High Affinity Binders
Engineering Proteins by Combining Deep Mutational Scanning and Yeast Display
Engineering Binders with Exceptional Selectivity
Affinity and Stability Analysis of Yeast Displayed Proteins
Antibody Library Screening Using Yeast Biopanning and Fluorescence-Activated Cell Sorting
A Hybrid Adherent/Suspension Cell-Based Selection Strategy for Discovery of Antibodies Targeting Membrane Proteins
Ligand Selection by Combination of Recombinant and Cell Panning Selection Techniques
Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display
Guidelines, Strategies, and Principles for the Directed Evolution of Cross-Reactive Antibodies Using Yeast Surface Display Technology
Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors
Yeast Display Guided Selection of pH Dependent Binders
Yeast Mating as a Tool for Highly Effective Discovery and Engineering of Antibodies via Display Methodologies
Humanization of Chicken-Derived Antibodies by Yeast Surface Display
Engineering Tissue Inhibitors of Metalloproteinases Using Yeast Surface Display
Discovery of Cyclic Peptide Binders from Chemically Constrained Yeast Display Libraries
Generation of Thermally Stable Affinity Pairs for Sensitive, Specific Immunoassays
Isolating Anti-Amyloid Antibodies from Yeast-Displayed Libraries
Engineering Proteins Containing Noncanonical Amino Acids on the Yeast Surface
Construction of Yeast Display Libraries for Selection of Antigen-Binding Variants of Large Extracellular Loop of CD81, a Major Surface Marker Protein of Extracellular Vesicles
Isolating and Engineering Fluorescence-Activating Proteins Using Yeast Surface Display
Simultaneous Display of Multiple Kinds of Enzymes on the Yeast Cell Surface for Multistep Reactions.
Subjects
Also listed under
SpringerLink (Online service)
Bookmark As
Citation
