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Plant Proteases and Plant Cell Death Methods and Protocols
Title
Plant Proteases and Plant Cell Death [electronic resource] : Methods and Protocols / edited by Marina Klemenčič, Simon Stael, Pitter F. Huesgen.
ISBN
9781071620793
Edition
1st ed. 2022.
Publication
New York, NY : Springer US : Imprint: Humana, 2022.
Physical Description
1 online resource (XI, 299 p.) 58 illus., 53 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume presents current methods to detect and measure the activity of proteolytic enzymes in organisms ranging from unicellular algae to flowering plants. Chapters detail in vitro production, characterization of plant proteases, tools for in vivo modifications, proteomic approaches for identification of substrates, inhibitors and interacting partners, and the function of plant proteases in plant programmed cell death. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Proteases: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. .
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Springer protocols (Series)
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Format
Books / Online
Language
English
Added to Catalog
June 21, 2022
Series
Methods in Molecular Biology, 2447
Contents
Expression and purification of the type I metacaspase from a cryptophyte Guillardia theta, GtMCA-I
Expression and purification of the type II metacaspase from a unicellular green alga Chlamydomonas reinhardtii
Expression in Escherichia coli, refolding, and purification of plant aspartic proteases
Production of functional plant legumain proteases using the Leishmania tarentolae expression system
Purification of His-tagged proteases from the apoplast of agroinfiltrated N. benthamiana
Identification of cognate protease/substrate pairs by use of class-specific inhibitors
Peptide backbone modifications for the assessment of cleavage site relevance in precursors of signaling peptides
Detection of apoplastic protease inhibitors using convolution activity based proteome profiling
Cysteine reactivity profiling to unveil redox regulation in phytopathogens
Determination of caspase-like activities in roots by the use of fluorogenic substrates
Detection of damage-activated metacaspase activity by Western blot in plants
Sensitive plant N-terminome profiling with HUNTER
Profiling sequence specificity of proteolytic activities using proteome-derived peptide libraries
Monitoring Cell Death via Ion leakage and PAM Fluorometry
Measuring and perturbing ferroptosis in plants
Detection and quantification of the hypersensitive response cell death in Arabidopsis thaliana
Monitoring of ATG4 protease activity during autophagy in the model microalga Chlamydomonas reinhardtii
Oxidative stress in roots - detection of lipid peroxidation and total antioxidative capacity
Imaging of potassium and calcium distribution in plant tissues and cells to monitor stress response and programmed cell death
Monitoring Pseudomonas syringae growth in agroinfiltrated leaves: the 'agromonas' assay
Analysis of virus spread around the cell death zone at spatiotemporal resolution using confocal microscopy
A user guide to validation, annotation and evaluation of N-terminome datasets with MANTI
Exploring posttranslational modifications with the Plant PTM Viewer. .
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