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Deubiquitinases Methods and Protocols
Title
Deubiquitinases [electronic resource] : Methods and Protocols / edited by Julie Maupin-Furlow, Mariola J. Edelmann.
ISBN
9781071628034
Edition
1st ed. 2023.
Publication
New York, NY : Springer US : Imprint: Humana, 2023.
Physical Description
1 online resource (XIII, 300 p.) 62 illus., 49 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This book aims to advance the understanding of deubiquitinases (DUBs) and DUB-like enzymes. Chapters detail methods used to identify, classify,and biochemically characterize DUBs along with approaches that enable both the determination and alteration of DUB biological function.. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Deubiquitinases: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .
Variant and related titles
Springer protocols (Series)
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Printed edition:
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Format
Books / Online
Language
English
Added to Catalog
February 22, 2023
Series
Methods in Molecular Biology, 2591
Contents
Characterization of Deubiquitinase Catalytic State Using a Structure-Based Approach
Synthetic Diubiquitin Fluorogenic Substrates to Study DUBs
Fluorometric Characterization of DUB Activity: From Single Enzyme Reactions to Live Intact Cell
Using Activity-Based Proteomics for the Quantification of Deubiquitinases in Animal Tissue
Design and Synthesis of Ubiquitin-Based Chemical tools with Unnatural Amino Acids for Selective Detection of Deubiquitinases
High Throughput Assay for Characterizing Rpn11 Deubiquitinase Activity
Activity-Based Protein Profiling (ABPP) for Cellular Deubiquitinase (DUB) and Inhibitor Profiling at Deep and High-Throughput Levels
High-Throughput MALDI-TOF Mass-Spectrometry-based Deubiquitylating Enzyme Assay for Drug Discovery
Bioinformatical Approaches to The Discovery and Classification of Novel Deubiquitinases
Application of Archaea in Deubiquitinase-Like Enzyme Discovery and Activity Assay
Characterization of the Ubiquitin and ISG15 Deconjugase Activity of SARS-CoV-1 and 2 Papain-like Protease
Next-generation Phage Display to Identify Peptide Ligands of Deubiquitinases
Identification of Deubiquitinase Substrates in Xenopus Egg Extract
Identification of Deubiquitinase Substrates in Saccharomyces cerevisiae by Systematic Overexpression
Real-time Deubiquitination Assays using a Free-Ubiquitin Sensor
A Method to Monitor Activity of SARS-CoV-2 Nsp3 from Cells
Use of Deubiquitinase Fusion Proteins to Characterize Endocytic Trafficking in Yeast.
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