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Microcephaly Methods and Protocols
Title
Microcephaly [electronic resource] : Methods and Protocols / edited by Timothy Gershon.
ISBN
9781071627525
Edition
1st ed. 2023.
Publication
New York, NY : Springer US : Imprint: Humana, 2023.
Physical Description
1 online resource (X, 176 p.) 36 illus., 24 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume discusses the latest imaging and molecular techniques for studying microcephaly through neural progenitor proliferation, survival, and gene expression. All of the methods covered in this book use the mouse as a model system, and include cellular, metabolic, transcriptomic studies of neural progenitors, MR imaging studies of brain growth in 3-dimensions and developmentally-adapted studies of behavior in neonatal mice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Microcephaly: Methods and Protocols is a valuable resource for all researchers who want to expand their knowledge and understanding of this important and developing field.
Variant and related titles
Springer protocols (Series)
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Format
Books / Online
Language
English
Added to Catalog
February 22, 2023
Series
Methods in Molecular Biology, 2583
Contents
Dissociation of Cerebellar Granule Neuron Progenitors for Culture, FACS, Transcriptomics, and Molecular Biology
Maintaining Cerebellar Granule Neuron Progenitors in Cell Culture
Proliferation Analysis of Cerebellar Granule Neuron Progenitors for Microcephaly Research, Using Immunofluorescent Staining and Flow Cytometry
Conventional and Spectral Karyotyping of Murine Cerebellar Granule Neuron Progenitors
Processing of Neonatal Mouse Brains for Immunohistochemical Analysis, As Required for Including Spindle Orientation Analysis and Analysis of DNA Damage and Apoptosis
Automated Immunofluorescence Staining for Analysis of DNA Damage and Apoptosis in Brain Sections
Automated Immunofluorescence Staining for Analysis of Mitotic Stages and Division Orientation in Brain Sections
scRNA-seq for Microcephaly Research [1]: Single-Cell Droplet Encapsulation, mRNA Capture, and cDNA Synthesis
scRNA-seq for Microcephaly Research [2]: Preparation of Single-Cell cDNA Libraries
scRNA-seq for Microcephaly Research [3]: Computational Analysis of scRNA-seq Data
scRNA-seq for Microcephaly Research [4]: Dirichlet Regression for Single Cell Population Differences
Mouse Brain MRI, Including In Vivo, Ex Vivo, and fcMRI for the Study of Microcephaly
Whole-Body Mouse Fluxomic Analysis to Detect Metabolic Disruptions Associated with Microcephaly, using 13C Isotopes
Neonatal Behavioral Screen for Mouse Models of Neurodevelopmental Disorders. .
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