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Emerging Model Organisms
Title
Emerging Model Organisms [electronic resource] / edited by Wei Wang, Nicolas Rohner, Yongfu Wang.
ISBN
9781071628751
Edition
1st ed. 2023.
Publication
New York, NY : Springer US : Imprint: Humana, 2023.
Physical Description
1 online resource (XV, 359 p.) 95 illus., 94 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume discusses different approaches towards understanding the brain plasticity, aging, and regeneration of the nervous system in model organisms. Chapters guide the reader through an overview of model organisms, setup of an aquatic system, Tol2-mediated transgenesis, CRISPR/Cas9-mediated gene knock-out and knock-in, Cas13d-mediated gene knockdown, tissue clearing and expansion methods for brain and spinal cord imaging, Histological techniques, chromatin Immunoprecipitation Sequencing (ChIP-Seq) analysis, single-cell RNA sequencing analysis, BrdU/EdU incorporation assays, brain and spinal cord injury in African killifish, and emerging technologies and future perspectives in African killifish research. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Emerging Model Organisms aims to be a useful practical guide to researches to help further their study in this field. .
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Format
Books / Online
Language
English
Added to Catalog
February 22, 2023
Series
Neuromethods, 194
Contents
Establish a fishroom for African turquoise killifish Nothobranchius furzeri
Gene editing and transgenic reporter assays in African killifish
Spinal cord injury in African killifish
Whole brain clearing and immunolabelling in the African killifish N. furzeri
Studying Mating Behaviors in Nothobranchius furzeri
A chromatin immunoprecipitation (ChIP) protocol for African turquoise killifish
EdU labeling of proliferating cells in the African killifish Nothobranchius furzeri
Immunofluorescence staining on cryosections with high autofluorescence in African killifish
Rapid visualization of gene expression using chromogenic RNA in situ hybridization in African killifish
Profiling gene expression in African turquoise killifish Nothobranchius furzeri embryos with RNA fluorescence in situ hybridization chain reaction (HCR)
Cell dissociation of tissues for single-cell analysis in African killifish
Cryopreservation of the Mexican Tetra: Astyanax mexicanus
Whole-mount multicolor fluorescent labeling by in situ hybridization in Astyanax mexicanus embryos and larvae
Application of CRISPR-Cas9 for functional analysis in A. mexicanus
Immunohistochemistry and in vivo neural imaging in A. mexicanus
Sleep behavior analysis in Astyanax mexicanus
A new method to analyze non-visual based social-like interactions in asocial cavefish
A generalist's guide to quantifying swimming behavior within traditional and non-traditional teleost models
Recording acoustic behaviour in Astyanax mexicanus fish:acquisition, decryption, interpretation
Generation of mini-brains from hiPSCs
Generating Human Pluripotent Stem Cell-Derived AssemBloids™ to Model Interneuron Migration and Immune Cell Interactions
Multiplexed quantitative proteomics analysis of developing human brain organoids.
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