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Vectorology for Optogenetics and Chemogenetics
Title
Vectorology for Optogenetics and Chemogenetics [electronic resource] / edited by Mark A.G. Eldridge, Adriana Galvan.
ISBN
9781071629185
Edition
1st ed. 2023.
Publication
New York, NY : Springer US : Imprint: Humana, 2023.
Physical Description
1 online resource (XIII, 333 p.) 71 illus., 64 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume provides an overview of the viral vectors and how they are applied to chemogenetic and optogenetic tools to the study of neural circuits. The chapters in this book are organized into three parts detailing (1) viral vectors, (2) vector modifications and applications, and (3) practical advice for the delivery of viral vectors, verification of injection accuracy, and monitoring of transgene expression. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Vectorology for Optogenetics and Chemogenetics aims to be a useful practical guide to researches to help further their study in this field.
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Format
Books / Online
Language
English
Added to Catalog
February 22, 2023
Series
Neuromethods, 195
Contents
Production, testing and verification of Lentivirus for regional targeting in the old-world monkey brain
HiRet/NeuRet vectors: Lentiviral system for highly efficient gene transfer through retrograde axonal transport.-Generation of High-Titer Defective HSV-1 Amplicon Vectors
Generation and Application of Engineered Rabies Viral Vectors for Neural Circuit Research
Quality control for Adeno-associated viral vector production
Vector tropism.-Viruses for systemic delivery
Transcriptomic Definition of Neuron Types
Enhancers for selective targeting
Pathway-selective reversible perturbations using a double-infection technique in the macaque brain
Pathway-Specific Chemogenetic Manipulation by Applying Ligand to Axonally-Expressed DREADDs
Convection-enhanced delivery of viral vectors
Multi-channel microinjector arrays for efficient viral vector delivery into rhesus monkey brain
Methods to verify expression and function of DREADDs using PET
Reporter selection and post-mortem methods to verify transgene expression
Considerations for the use of viral vectors in NHP experiments.
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