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Axon Regeneration Methods and Protocols
Title
Axon Regeneration [electronic resource] : Methods and Protocols / edited by Ava J. Udvadia, James B. Antczak.
ISBN
9781071630129
Edition
1st ed. 2023.
Publication
New York, NY : Springer US : Imprint: Humana, 2023.
Physical Description
1 online resource (XII, 451 p.) 70 illus., 65 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume covers a wide range of approaches utilized to decipher cellular and molecular mechanisms that contribute to successful nerve regeneration leading to functional recovery. Chapters detail a variety of models utilizing both in vivo and in vitro approaches, physical injury models, methods for the isolation/analysis of various macromolecules, live and fixed imaging of regenerating axons, and for quantifying behavioral endpoints enable measurements of regenerative success. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Axon Regeneration: Methods and Protocols aims to be comprehensive guide for researchers.
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Springer protocols (Series)
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Format
Books / Online
Language
English
Added to Catalog
April 05, 2023
Series
Methods in Molecular Biology, 2636
Contents
Defining selective neuronal resilience and identifying targets for neuroprotection and axon regeneration using single-cell RNA sequencing: Experimental approaches
Defining selective neuronal resilience and identifying targets of neuroprotection and axon regeneration using single-cell RNA sequencing - computational approaches
Retinal Ganglion Cell Axon Fractionation
Analysis of Immediate Early Gene Expression Levels to Interrogate Changes In Cortical Neuronal Activity Patterns Upon Vision Loss
Non-coding regulatory RNAs: Isolation and analysis of neuronal circular RNAs and microRNAs
Transneuronal delivery of cytokines to stimulate mammalian spinal cord regeneration
Epigenomic Profiling of Dorsal Root Ganglia Upon Regenerative and Non-Regenerative Axonal Injury
Profiling locally translated mRNAs in regenerating axons
Analysis of axonal regrowth and dendritic remodeling after optic nerve crush in adult zebrafish
Assaying Optic Nerve Regeneration in Larval Zebrafish
Surgical Methods in Postmetamorphic Xenopus laevis: Optic Nerve Crush Injury Model
Generating widespread and scalable retinal lesions in adult zebrafish by intraocular injection of ouabain
A reproducible spinal cord crush injury in the regeneration permissive axolotl
Live imaging of axonal dynamics after laser axotomy of peripheral neurons in zebrafish
Rapid testing of gene function in axonal regeneration after spinal cord injury using larval zebrafish
Translational Ribosomal Affinity Purification (TRAP) and Bionformatic RNA-Seq Analysis in Postmetamorphic Xenopus laevis
LCM-seq for Retinal Cell Layer-Specific Responses During Optic Nerve Regeneration
Temporal-Based Dynamic Profiling of DNA Accessibility With ATAC-Seq in the Regenerating Retina of Zebrafish
Quantitative Proteomics of Nervous System Regeneration: From Sample Preparation to Functional Data Analyses
Live Cell Imaging of Dynamic Processes in Adult Zebrafish Retinal Cross-section Cultures
PCNA staining of retinal cryosections to assess microglial/macrophage proliferation
Using a Drosophila injury model to investigate RNA repair and splicing in axon regeneration
Assessing rewiring of the retinal circuitry after inner retinal lesion zebrafish
Analysis of visual recovery after optic nerve crush in adult zebrafish.
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