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Protocols for Neural Cell Culture

Title
Protocols for Neural Cell Culture [electronic resource] / edited by Laurie C. Doering.
ISBN
9781607612926
Edition
4th ed. 2010.
Publication
Totowa, NJ : Humana Press : Imprint: Humana, 2010.
Physical Description
1 online resource (XII, 400 p.) 85 illus., 14 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies. The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience. While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death. With its detailed, step-by-step laboratory protocols, this volume provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the previous editions' tradition of exceptional quality and thorough coverage in tissue culture protocols for today's neuroscience in order to inspire researchers in the field to further explore this imperative area of study.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
August 14, 2023
Series
Springer Protocols Handbooks,
Springer Protocols Handbooks,
Contents
Neurosphere and Neural Colony-Forming Cell Assays
Directed Neuronal Differentiation of Embryonic and Adult-Derived Neurosphere Cells
Culture and Differentiation of Human Neural Stem Cells
Neural Differentiation of Human Embryonic Stem Cells
Isolation and Culture of Primary Human CNS Neural Cells
Bioengineering Protocols for Neural Precursor Cell Expansion
Intracellular Calcium Assays in Dissociated Primary Cortical Neurons
Dissociated Hippocampal Cultures
Primary Sensory and Motor Neuron Cultures
Retinal Cell and Tissue Culture
Preparation of Normal and Reactive Astrocyte Cultures
Oligodendrocyte Progenitor Cell Culture
Isolation of Microglia Subpopulations
Microglia from Progenitor Cells in Mouse Neopallium
Primary Schwann Cell Cultures
Primary Dissociated Astrocyte and Neuron Co-culture
Cerebellar Slice Cultures
Hippocampal Slice Cultures
Molecular Substrates for Growing Neurons in Culture
Guidance and Outgrowth Assays for Embryonic Thalamic Axons
Detection of Cell Death in Neuronal Cultures
Live Imaging of Neural Cell Functions
Tissue Culture Procedures and Tips.
Also listed under
Doering, Laurie C. editor.
SpringerLink (Online service)
Citation

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