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Drosophila Methods and Protocols
Title
Drosophila [electronic resource] : Methods and Protocols / edited by Christian Dahmann.
ISBN
9781071625415
Edition
3rd ed. 2022.
Publication
New York, NY : Springer US : Imprint: Humana, 2022.
Physical Description
1 online resource (XI, 424 p.) 74 illus., 69 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of tissue mechanics, AFM measurements, sample preparation for electron microscopy, live imaging of different tissues and organs, and quantitative image analysis. Written in the format of the highly successful Methods in Molecular Biology series, chapters include an introduction to their topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Drosophila: Methods and Protocols, Third Edition serves as a useful and practical guide to new researchers and experts using Drosophila as a model system. Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Variant and related titles
Springer protocols (Series)
Other formats
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Printed edition:
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Format
Books / Online
Language
English
Added to Catalog
August 14, 2023
Series
Methods in Molecular Biology, 2540
Contents
Using FlyBase, a database of Drosophila genes and genetics
The Q-System: A versatile repressible binary expression system
Resources and methods for the analysis of microRNA function in Drosophila
Analysis of single-cell transcriptome data in Drosophila
Prime editing for precise genome engineering in Drosophila
CRISPR/Cas9 mediated precise and efficient genome editing in Drosophila
Tissue-specific CRISPR-Cas9 screening in Drosophila
CRISPR-based transcriptional activation in Drosophila
Tracing and manipulating Drosophila cell lineages based on CRISPR: CaSSA and CLADES
Studying protein function using nanobodies and other protein binders in Drosophila
Anchor Away: A system for fast inhibition of proteins in Drosophila
Tagging Drosophila proteins with genetically-encoded fluorophores
Optogenetic methods to control tissue mechanics in Drosophila
Optochemical control of cell contractility in Drosophila embryos
Stiffness measurement of Drosophila egg chambers by atomic force microscopy
Cultivation and live imaging of Drosophila imaginal discs
Sample preparation and imaging of the pupal Drosophila abdominal epidermis
FlyClear: A tissue clearing technique for high-resolution microscopy of Drosophila
Preparation of Drosophila tissues and organs for transmission electron microscopy
Segmentation and quantitative analysis of epithelial tissues
Genetically-encoded sensors to study metabolism in Drosophila.
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