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Protein-Protein Interactions Methods and Protocols
Title
Protein-Protein Interactions [electronic resource] : Methods and Protocols / edited by Shahid Mukhtar.
ISBN
9781071633274
Edition
1st ed. 2023.
Publication
New York, NY : Springer US : Imprint: Humana, 2023.
Physical Description
1 online resource (XV, 486 p.) 1 illus.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This detailed volume provides a comprehensive collection of classic and cutting-edge methods and techniques in mapping protein-protein interactions. The chapters include a variety of in vitro and in vivo experimental methods covering cell biology, biochemistry, and biophysics. In addition, the book also explores in silico methods including sequence-, structure-, and phylogenetic profile-based approaches as well as gene expression and machine learning methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein-Protein Interactions: Methods and Protocols serves as an ideal guide for researchers working in protein science and beyond.
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Springer protocols (Series)
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Format
Books / Online
Language
English
Added to Catalog
August 14, 2023
Series
Methods in Molecular Biology, 2690
Contents
Yeast-Two-Hybrid Technique to Identify Protein-Protein Interactions
Cytotrap: An Innovative Approach for Protein-Protein Interaction Studies for Cytoplasmic Proteins
Analyzing Protein-Protein Interactions Using the Split-Ubiquitin System
Detection of Protein-Protein Interactions Utilizing the Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System
Preparation and Utilization of a Versatile GFP-Protein Trap-Like System for Protein Complex Immunoprecipitation in Plants
Tandem Affinity Purification (TAP) of Interacting Prey Proteins with FLAG- and HA-Tagged Bait Proteins
Affinity Purification-Mass Spectroscopy (AP-MS) and Co-Immunoprecipitation (Co-IP) Technique to Study Protein-Protein Interactions
Co-Immunoprecipitation-Based Identification of Effector-Host Protein Interactions from Pathogen-Infected Plant Tissue
Co-Immunoprecipitation for Assessing Protein-Protein Interactions in Agrobacterium-Mediated Transient Expression System in Nicotiana benthamiana
Detection of Protein-Protein Interactions Using Glutathione-S-Transferase (GST) Pull-Down Assay Technique
Bimolecular Fluorescence Complementation (BiFC) Assay to Visualize Protein-Protein Interactions in Living Cells
Detecting Protein-Protein Interactions Using Bimolecular Fluorescence Complementation (BiFC) and Luciferase Complementation Assays (LCA)
Forster Resonance Energy Transfer (FRET) to Visualize Protein-Protein Interactions in the Plant Cell
Dynamic Proximity Tagging in Living Plant Cells with Pupylation-Based Interaction Tagging
Characterization of Small Molecule-Protein Interactions Using SPR Method
High-Throughput Protein-Protein Interactions Screening Using Pool-Based Liquid Yeast Two-Hybrid Pipeline
Dynamic Enrichment for Evaluation of Protein Networks (DEEPN): A High Throughput Yeast Two-Hybrid (Y2H) Protocol to Evaluate Networks
An Interactome Assay for Detecting Interactions between Extracellular Domains of Receptor Kinases
Next-Generation Yeast Two-Hybrid Screening to Discover Protein-Protein Interactions
Bioinformatic Analysis of Yeast Two-Hybrid Next-Generation Interaction Screen Data
Discovering Protein-Protein Interactions Using Co-Fractionation-Mass Spectrometry with Label-Free Quantitation
Protein-Protein Interaction Network Mapping by Affinity Purification Cross-Linking (AP-CXL-MS) Proteomics
Protein Interaction Screen on a Peptide Matrix (PrISMa)
Analyzing Protein Interactions by MAC-Tag Approaches
Identification and Quantification of Affinity-Purified Proteins with MaxQuant, Followed by the Discrimination of Nonspecific Interactions with the CRAPome Interface
Cataloguing Protein Complexes In Planta Using TurboID-Catalyzed Proximity Labeling
A Data-Driven Signaling Network Inference Approach for Phosphoproteomics
Pairwise and Multi-Chain Protein Docking Enhanced Using LZerD Web Server
Predicting Protein Interaction Sites Using PITHIA
Using PlaPPISite to Predict and Analyze Plant Protein-Protein Interaction Sites
Machine Learning Methods for Virus-Host Protein-Protein Interaction Prediction
Protein-Protein Interaction Network Exploration Using Cytoscape
Search, Retrieve, Visualize, and Analyze Protein-Protein Interactions from Multiple Databases: A Guide for Experimental Biologists
Centrality Analysis of Protein-Protein Interaction Networks Using R
Protein-Protein Interaction Network Analysis Using NetworkX
Building Protein-Protein Interaction Graph Database Using Neo4j.
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