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Mammalian Synthetic Systems
Title
Mammalian Synthetic Systems [electronic resource] / edited by Francesca Ceroni, Karen Polizzi.
ISBN
9781071637180
Edition
1st ed. 2024.
Publication
New York, NY : Springer US : Imprint: Humana, 2024.
Physical Description
XI, 316 p. 88 illus.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume details cutting-edge methods that enables engineering of novel functions in mammalian cells. Chapters guide readers through cell-to-cell communication, cell fate control, protein and RNA-based biosensors together with tools for more reliable and faster mammalian genome editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammalian Synthetic Systems aims to ensure successful results in the further study of this vital field.
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Springer protocols (Series)
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Format
Books / Online
Language
English
Added to Catalog
April 10, 2024
Series
Methods in Molecular Biology, 2774
Contents
A directed evolution protocol for engineering minimal transcription factors, based on CIS display
Set-up and applications of Modular Protein Expression Toolboxes (MoPET) for mammalian systems
Coiled-coiled interaction toolbox for engineering mammalian cells
A mammalian-based synthetic biology toolbox to engineer membrane-membrane interfaces
Engineered protease-responsive RNA Binding Proteins (RBPs) to expand the toolbox of synthetic circuits in mammalian cells
Mechanistic model-driven biodesign in mammalian synthetic biology
Realizing Antithetic Integral Feedback Control in Mammalian Cells
A computational modeling approach for the design of genetic control systems that respond to transcriptional activity
Flux Balance Analysis of Mammalian Cell Systems
Multiplex functional characterization of protein variant libraries in mammalian cells with single-copy genomic integration and high throughput DNA sequencing
Flow Cytometry Quantification of Transient Transfections in Mammalian CellsRNA switches using Cas proteins
Multiplexed transactivation of mammalian cells using dFnCas12a-VPR
Anti-CRISPR Proteins and their Application to Control CRISPR-effectors in Mammalian Systems
Post-translational Remote Control Mediated by Physical Inducers for Rapid Protein Release in Engineered Mammalian Cells
Detection of microRNAs Using Synthetic Toehold Switch in Mammalian Cells
Detection of microRNAs Using Synthetic Toehold Switch in Mammalian Cells
High-throughput spectroscopic analysis of mRNA capping level
In vitro generation of megakaryocytes from engineered mouse embryonic stem cells
Preparation of chromobodies for the detection of cell surface epitopes.
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