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Cerebral Cortex Development Methods and Protocols
Title
Cerebral Cortex Development [electronic resource] : Methods and Protocols / edited by Koh-ichi Nagata.
ISBN
9781071638101
Edition
1st ed. 2024.
Publication
New York, NY : Springer US : Imprint: Humana, 2024.
Physical Description
1 online resource (XII, 355 p.) 101 illus., 77 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume explores the latest developments in the study of the cerebral cortex. The chapters in this book cover a wide array or established methods such as in situ hybridization of brain slices; two-photon FRET/FLIM imaging of cerebral neurons; BioID analysis of actin-binding proteins; generation of iPS cells using Sendai Virus vectors; in vivo whole-cell recording from the mouse brain; and locomotor assays in Drosphila larvae and adult flies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Cerebral Cortex Development: Methods and Protocols is a valuable resource that will help both expert and novice researchers further enhance their understanding of this important field.
Variant and related titles
Springer protocols (Series)
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Printed edition:
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
May 08, 2024
Series
Methods in Molecular Biology, 2794
Contents
Overview: Research on the Genetic Architecture of the Developing Cerebral Cortex in Norms and Diseases
In Situ Hybridization of Brain Slices
Immunohistochemistry of Brain Tissue
Two-Photon FRET/FLIM Imaging of Cerebral Neurons
Pre- and Post-Embedding Immunoelectron Microscopy to Analyze the Central Nervous System
Mouse Brain Tissue Preparation for Scanning Electron Microscopy
Simple Method for the Preparation of the Postsynaptic Density Fraction from Mouse Brain
Reconstitution of Neuronal Motor Traffic on Septin-Associated Microtubules
BioID Analysis of Actin-Binding Proteins
Proteomic Analyses of the Mouse Brain
Generation of iPS Cells using Sendai Virus Vectors
Directed Differentiation of Neurons from Human iPSCs for Modeling Neurological Disorders
Generating Homogeneous Brain Organoids from Human iPSCs
Primary Culture of Dissociated Neurons from Embryonic Cerebral Cortex
Immunocytochemistry of Primary Cultured Cerebral Cortical Neurons
Brain Sample Preparation after Performing In Utero Electroporation to Proliferating and Differentiated Cells in the Developing Mouse Neocortex
Live Imaging of Migrating Neurons and Glial Progenitors Visualized by In Utero Electroporation
Ca2+ Imaging in Immature Cortical Neurons
In Vitro Patch-Clamp
In Vivo Whole-Cell Recording from the Mouse Brain
In Vivo Brain Microdialysis
CT-Scan Data Analysis in Malformations of Cortical Development
MRI Data Analysis in Malformations of Cortical Development
Genomic Copy Number Analysis using Droplet Digital PCR: A Simple Method with EvaGreen Single-Color Fluorescent Design
Locomotor Assays in Drosophila Larvae and Adult Flies
Analyses of Genetic Regulation of the Nervous System in the Nematode Caenorhabditis elegans
Analyses of Neural Circuits Governing Behavioral Plasticity in the Nematode Caenorhabditis elegans
Social Behavior in Animal Models of Autism Spectrum Disorder
Preparation of Healthy Single Neuron or Astrocyte Suspension from Adult Mouse Brain for RNA-seq.
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