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Medical biotechnology
Author
Title
Medical biotechnology / Bernard R. Glick, Department of Biology, University of Waterloo, Waterloo, Ontario, Canada, Terry L. Delovitch, Department of Microbiology and Immunology, Western University, London, Ontario, Canada, Cheryl L. Patten, Biology Department, University of New Brunswick, Fredericton, New Brunswick, Canada.
ISBN
9781555818890
1555818897
9781680159301
1680159305
9781555817053
155581705X
Publication
Washington, DC : ASM Press, [2014]
Copyright Notice Date
©2014
Physical Description
1 online resource (758 pages) : color illustrations
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
The future is now-this groundbreaking textbook illustrates how biotechnology has radically changed the way we think about health care.
Variant and related titles
Knovel. OCLC KB.
Other formats
Print version: Glick, Bernard R. Medical biotechnology. Washington, DC : ASM Press, [2014]
Format
Books / Online
Language
English
Added to Catalog
October 02, 2023
Bibliography
Includes bibliographical references and index.
Contents
Machine generated contents note: SECTION I The Biology behind the Technology
1. Fundamental Technologies
Molecular Cloning
Preparation of DNA for Cloning
Insertion of Target DNA into a Plasmid Vector
Transformation and Selection of Cloned DNA in a Bacterial Host
Cloning Eukaryotic Genes
Recombinational Cloning
Genomic Libraries
Amplification of DNA Using PCR
DNA Sequencing Technologies
Dideoxynucleotide Procedure
Pyrosequencing
Sequencing Using Reversible Chain Terminators
Sequencing by Ligation
Sequencing Whole Genomes
Shotgun Cloning Strategy
High-Throughput Next-Generation Sequencing Strategies
Genomics
Transcriptomics
Proteomics
Metabolomics
Summary
Review Questions
References
2. Fundamental Concepts in Immunology
The Immune Response
Overview of Infection and Immunity
Functions of the Immune System
Innate Immunity
Adaptive Immunity
Cells of the Immune System: Cell-Mediated Immunity.
Note continued: Bone Marrow Precursor Cells
Myeloid Cells Mediate Innate Immunity
Lymphocytes
Antigen-Presenting Cells
Effector Cells
Tissues of the Immune System
Peripheral Lymphoid Organs
Lymphocyte Recirculation and Migration into Tissues
Antigen Recognition by T Cells
Humoral Immunity
Structure of Immunoglobulins
Functions of Immunoglobulins
Types of Antibodies: Applications
Polyclonal Antibodies
Antisera
Monoclonal Antibodies
Recombinant Antibodies
Immunological Techniques
Enzyme-Linked Immunosorbent Assay
Enzyme-Linked Immunospot Assay
Flow Cytometry
Mass Cytometry
Two-Photon Intravital Cell Imaging
Summary
Review Questions
References
3. The Genetic Basis of Disease
Chromosomal Disorders and Gene Mapping
Chromosomes and Chromosome Abnormalities
Human Genome Mapping
Genome-Wide Association Studies
Single-Gene Disorders
Mode of Inheritance
Thalassemia.
Note continued: Sickle-Cell Anemia
Hemophilia
Cystic Fibrosis
Tay-Sachs Disease
Fragile X Syndrome
Huntington Disease
Polygenic Disorders and Gene Clustering
GWAS Strategies To Map Genes for Polygenic Disease
Breast Cancer
Alzheimer Disease
Type 1 Diabetes
Cardiovascular Disease
Mitochondrial Disorders
Disorders
Genetics
Mitochondrial Homeostasis and Parkinson Disease
Prevalence
Diagnosis and Prognosis
Treatment
Summary
Review Questions
References
4. Immune Pathogenesis
Models of Immune System Lesions
Immunological Tolerance
Failure of Immune Tolerance and Development of Autoimmune Disease
Immune Surveillance against Tumors
Immune Evasion by Tumors
Inflammation and Immune Hypersensitivity Disorders
Types of Inflammation and Associated Immune Hypersensitivity Reactions
Immediate Hypersensitivity
Therapy for Immediate Hypersensitivity.
Note continued: Antibody- and Antigen-Antibody Complex-Induced Disease
T-Cell-Mediated Diseases
Immunodeficiency Disorders and Defects in Development of the Immune System
Primary Immunodeficiencies
Secondary Immunodeficiencies
Aids
Summary
Review Questions
References
5. Microbial Pathogenesis
Introduction
Bacterial Infections
Attachment to Host Cells
Invasion and Dissemination
Evasion of Host Defenses and Proliferation
Damage to Host Tissues
Identification of Bacterial Virulence Factors
Evolution of Bacterial Pathogens
Treatment of Bacterial Infections
Viral Infections
Attachment and Entry
Viral Gene Expression and Replication
Virus Assembly and Release
General Patterns of Viral Infections of Humans
Targets for Treatment of Viral Infections
Summary
Review Questions
References
SECTION II Production of Therapeutic Agents
6. Modulation of Gene Expression.
Note continued: Manipulating Gene Expression in Prokaryotes
Promoters
Translational Regulation
Codon Usage
Protein Stability
Fusion Proteins
Metabolic Load
Chromosomal Integration
Increasing Secretion
Overcoming Oxygen Limitation
Reducing Acetate
Protein Folding
Heterologous Protein Production in Eukaryotic Cells
Eukaryotic Expression Systems
Saccharomyces cerevisiae Expression Systems
Other Yeast Expression Systems
Baculovirus
Insect Cell Expression Systems
Mammalian Cell Expression Systems
Directed Mutagenesis
Oligonucleotide-Directed Mutagenesis with M13 DNA
Oligonucleotide-Directed Mutagenesis with Plasmid DNA
PCR-Amplified Oligonucleotide-Directed Mutagenesis
Error-Prone PCR
Random Mutagenesis
DNA Shuffling
Examples of Modified Proteins
Summary
Review Questions
References
7. Genetic Engineering of Plants
Plant Transformation with the Ti Plasmid of A. tumefaciens.
Note continued: Physical Transfer of Genes to Plants
Chloroplast Engineering
Transient Gene Expression
Molecular Pharming
Therapeutic Agents
Antibodies
Edible Vaccines
Summary
Review Questions
References
SECTION III Diagnosing and Treating Human Disease
8. Molecular Diagnostics
Immunological Approaches To Detect Protein Biomarkers of Disease
Enzyme-Linked Immunosorbent Assays
Measuring Disease-Associated Proteins by Sandwich ELISA
Diagnosing Autoimmune Diseases by an Indirect ELISA
Immunoassays for Infectious Disease
Protein Arrays To Detect Polygenic Diseases
Immunoassays for Protein Conformation-Specific Disorders
DNA-Based Approaches to Disease Diagnosis
Hybridization Probes
Allele-Specific Hybridization
Oligonucleotide Ligation Assay
Padlock Probes
Allele-Specific PCR
TaqMan PCR
Real-Time PCR To Detect Infectious Disease.
Note continued: Detection of Multiple Disease-Associated Mutations Using Microarrays
Detection of Epigenetic Markers
Detection of SNPs by Mass Spectrometry
Detecting RNA Signatures of Disease
Detection of Disease-Associated Changes in Gene Expression Using Microarrays
Detection of RNA Signatures of Antibiotic Resistance in Human Pathogens
Detection of miRNA Signatures of Cancers
Summary
Review Questions
References
9. Protein Therapeutics
Pharmaceuticals
Interferon
Human Growth Hormone
Tumor Necrosis Factor
Targeting Mitochondria
Extending Protein Half-Life
Engineered Bacteriophages
Recombinant Antibodies
Preventing Rejection of Transplanted Organs
Hybrid Human
Mouse Monoclonal Antibodies
Human Monoclonal Antibodies
Antibody Fragments
Combinatorial Libraries of Antibody Fragments
Anticancer Antibodies
Antianthrax Antibodies
Antiobesity Antibodies
Enhanced Antibody Half-Life
Enzymes.
Note continued: DNasel
Alginate Lyase
Phenylalanine Ammonia Lyase
[alpha]1-Antitrypsin
Glycosidases
Lactic Acid Bacteria
Interleukin-10
Leptin
An HIV Inhibitor
Insulin
Summary
Review Questions
References
10. Nucleic Acid Therapeutic Agents and Human Gene Therapy
Treating Genetic and Nongenetic Disorders
Targeting Specific mRNAs and DNAs
Antisense RNA
Aptamers
Ribozymes
DNAzymes
Interfering RNA
Zinc Finger Nucleases
Viral Delivery Systems
Gammaretrovirus
Lentivirus
Adeno-Associated Virus
Adenovirus
Herpes Simplex Virus 1
Nonviral Nucleic Acid Delivery Systems
Direct Injection
Lipids
Bacteria
Dendrimers
Antibodies
Aptamers
Transposons
Prodrug Activation Therapy
Gene Therapy
Severe Combined Immunodeficiency
Cancer
Eye Disorders
Muscle Disorders
Neurological Disorders
Summary
Review Questions
References
11. Vaccines.
Note continued: Vaccination: Overview
Advantages
Limitations
Current Vaccine Design
Subunit Vaccines
Herpes Simplex Virus
Cholera
Severe Acute Respiratory Syndrome
Staphylococcus aureus
Human Papillomavirus
Peptide Vaccines
Malaria
Cancer
Autoimmune Disease
Allergy
Dendritic Cell Vaccines
Human Immunodeficiency Virus
Cancer
DNA Vaccines
Delivery and Immune Mechanisms of Action
Advantages and Disadvantages
Improved Efficacy and Immunogenicity
Attenuated Vaccines
Cholera
Salmonella Species
Leishmania Species
Poliovirus
Influenza Virus
Dengue Virus
Vector Vaccines
Vaccines Directed against Viruses
Vaccines Directed against Bacteria
Bacteria as Antigen Delivery Systems
Adjuvants
Systems Biology and Evaluation of Vaccines
Summary
Review Questions
References
12. Societal Issues
Safety and Ethical Issues
Regulation of New Drugs.
Note continued: Regulation of Genetic and Genomic Testing
Patenting Biotechnology
Patenting
Patenting in Different Countries
Patenting DNA Sequences
Patenting Living Organisms
Patenting and Fundamental Research
Economic Issues
Summary
Review Questions
References.
Subjects (Medical)
Biomedical Technology
Biomedical Engineering
Biotechnology
Medical Laboratory Science
Genre/Form
Electronic books.
Electronic book.
Electronic books.
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