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Mucins Methods and Protocols
Title
Mucins [electronic resource] : Methods and Protocols / edited by Akihiko Kameyama.
ISBN
9781071636701
Edition
1st ed. 2024.
Publication
New York, NY : Springer US : Imprint: Humana, 2024.
Physical Description
1 online resource (XIV, 417 p.) 1 illus.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume explores the latest advancements in mucin research. The chapters in this book are organized into 8 parts and cover a wide range of topics such as mucin extraction, isolation, physicochemical property analysis, and experimental methods. The chapters also discuss the origins of mucins in jellyfish, feces, saliva and salivary glands, bronchi, stomach, and cervical tract; organic synthesis of peptides glycosylated at specific sites; analysis of mucin gene expression and methylation-specific electrophoresis of genes; imaging of mucin networks by AFM; and experimental methods using supported molecular matrix electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Mucins: Methods and Protocols is a valuable resource for researchers who want to obtain in-depth insight on how to more thoroughly conduct mucin research in their laboratories. .
Variant and related titles
Springer protocols (Series)
Other formats
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Format
Books / Online
Language
English
Added to Catalog
March 01, 2024
Series
Methods in Molecular Biology, 2763
Contents
Preparation of Jellyfish Mucins
Extraction of Mucin from Rodent Feces and Determination of O-Linked Oligosaccharide Chain Equivalent Derived from Fecal Mucin
Preparation of Soluble Mucin Solutions from the Salivary Glands
Isolation of Membrane Bound Mucins from Human Bronchial Epithelial Cells
Extraction and Fractionation of Human Gastric Mucins from Gastric Juice
Extraction of Mucins from the Mammalian Intestinal Tract
Supported Molecular Matrix Electrophoresis
Supported Immunohistochemical Staining of Mucins
Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride Membrane
Quantitation of Mucin by Densitometry of an Alcian Blue-Stained Membrane
Quantitation of MUC5AC and MUC5B by Stable Isotope Labeling Mass Spectrometry
Preparation of O-Glycans from Mucins using Hydrazine Treatment
Eliminative Oximation of O-glycans from Mucins
9-Fluorenylmethyl Chloroformate Labeling for O-Glycan Analysis
Liquid Chromatography and Capillary Electrophoresis Analysis of 2AA-Labeled O-Glycans
Preparation of Mucin Glycopeptides by Organic Synthesis
MALDI-TOF MS/MS Analysis of Permethylated O-Glycan Alditols Released from Mucins
Structural Elucidation of Sialylated O-Glycan Alditols Obtained from Mucins by Mass Spectrometry
Differential Glycoform Analysis of MUC1 Derived from Biological Specimens using an Antibody-Overlay Lectin Microarray
ISOGlyP: O-Glycosylation Site Prediction using Peptide Sequences and GALNTs
Assessment of Mucin-Associated Gene Expression Levels on the Ocular Surface
Methylation Specific Electrophoresis
Expression Analysis of Genes Corresponding to Mucins and their Glycans from Cervical Tissue using RNA Sequencing
Recombinant Production of Glycoengineered Mucins in HEK293-F Cells
Analysis of the Interaction between Mucin and Green Fluorescent Protein (GFP)-Tagged Galectin-2 using a 96-Well Plate
Solution NMR Analysis of O-Glycopeptide-Antibody Interaction
Cultivation of Microorganisms in Media Supplemented with Mucin Glycoproteins
Bacterial Enzyme Assay for Mucin Glycan Degradation
Measurement of Mucinase Activity
Adhesion Inhibition Assay for Helicobacter pylori to Mucin by lactobacillus
Imaging of Mucin Networks with Atomic Force Microscopy
Molecular Dynamics Simulation and Docking of MUC1 O-Glycopeptide
Fabrication and Characterization of Mucin Nanoparticles for Drug Delivery Applications
Evaluation of Rheological Properties of Saliva by Determining the Spinnbarkeit
Mechanical Characterization of Mucus on Intestinal Tissues by Atomic Force Microscopy.
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