9783527836840

3527836845

9783527836864

3527836861

9783527350322

Electronic reproduction. Hoboken, N.J. Available via World Wide Web.

Machine-generated record

Intro

Intracellular Thermometry with Fluorescent Molecular Thermometers

Preface

Contents

1 Temperature for Living Things

1.1 Temperature of Individuals

1.2 Responses to Temperature Variation at the Cellular Level

1.3 Significance of Intracellular Thermometry

References

2 Fluorescent Molecular Thermometers

2.1 The Basics of Fluorescence

2.2 Responses of Fluorescent Molecular Thermometers

2.3 Small Organic Molecules Involving Intersystem Crossing

2.4 Small Organic Molecules with a Rotating Substituent Group

2.5 Reactive Small Organic Molecules

2.6 Viscosity-Sensitive Small Organic Molecules

2.7 Other Small Organic Molecules

2.8 Organometallic Complexes

2.9 Excimers and Exciplexes

2.10 Host-guest Interactions

2.11 Synthetic Polymers

2.12 Nucleic Acids (DNA and RNA)

2.13 Peptides

2.14 Fluorescent Proteins

2.15 Non-covalent Systems Based on Thermo-responsive Self-assembly and an Environment-sensitive Fluorophore

2.16 Inorganic Nanomaterials

2.17 Hybrid Nanomaterials

References

3 Intracellular Thermometry with Fluorescent Molecular Thermometers

3.1 Early Attempts for Cellular Thermometry

3.2 Introduction of Fluorescent Molecular Thermometers into a Living Cell

3.3 Cytotoxicity Assessment

3.4 Practice of Intracellular Thermometry with Fluorescent Molecular Thermometers

3.5 Intracellular Thermometry with Organelle-targeted Fluorescent Molecular Thermometers

3.6 Intracellular Thermometry of Brown Adipocytes

3.7 Application of Intracellular Thermometry in Various Biological Fields

References

4 Cellular Thermometry Based on Non-fluorometric Principles

4.1 Infrared Thermometry

4.2 Photoacoustic Thermometry

4.3 Raman Thermometry

4.4 Use of Transmission Spectroscopy

4.5 Thermocouple

4.6 Resonant Thermal Sensor

4.7 Bimaterial Microcantilever

4.8 Thermistor

References

5 Reliability Issue in Intracellular Thermometry

5.1 Sensitivity and Temperature Resolution

5.2 Functional Independency of Fluorescent Molecular Thermometers

5.3 Preparation of a Calibration Curve of Fluorescent Molecular Thermometers

5.4 Objection to Endogenous Thermogenesis

5.5 Possible Artifacts in Near-infrared Luminescent Thermometry and Proposal for Reliable Thermometry

References

6 Applications of Intracellular Thermometry

6.1 Creation of New Biological Concepts

6.2 In vivo Temperature Measurements

6.3 Thermal Medicine at a Single-cell Level

6.4 Utilization of Nanoheater/Fluorescent Thermometer Hybrids in Biotechnology

6.5 Accurate Measurements of Absolute Intracellular Temperature

6.6 Simultaneous Monitoring of Intracellular Temperature and the Concentration of a Chemical Species Related to a Temperature Variation Inside a Living Cell

6.7 Intracellular Thermometry of Plant Cells

References