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Practical Handbook on Soil Protists

Practical Handbook on Soil Protists [electronic resource] / edited by N. Amaresan, Komal A. Chandarana.
1st ed. 2024.
New York, NY : Springer US : Imprint: Humana, 2024.
Physical Description
XII, 205 p. 19 illus., 13 illus. in color.
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This volume details a wide range of basic and advanced techniques associated with research on isolation of important soil protozoa, and identification. Chapters guide readers through calculations of soil protists, basic techniques for isolation, enumeration, enrichment, molecular techniques, and staining techniques. Authoritative and cutting-edge, Practical Handbook on Soil Protists aims to ensure successful results in the further study of this vital field.
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April 10, 2024
Springer protocols (Series)
Springer Protocols Handbooks,
Soil sampling, processing and storage
Initial observation of protist from soil
Abundance calculation of naked and testate amoebae from soil
Abundance calculation of mycophagous amoebae
Abundance calculation of ciliates from soilAbundance calculation of flagellates from soil
Methodology to study species diversity of naked amoeba and testate amoeba
Non-flooded Petri plate method to study the species diversity of flagellates
Enumeration of testate amoeba through direct count from soil
Most Probable Number (MPN) method for enumeration of soil protists
Standard solutions and media used for isolation of soil protists
Isolation techniques for amoeba
Isolation of ciliates and flagellates from soil
Isolation and Enumeration of Mycophagous Protist
Media used for enrichment of soil protists
Enrichment of naked amoebae species
Enrichment of ciliates and flagellates
Enrichment of mycophagous protists
Mono-axenic cultivation of protists
Axenic cultivation of soil protists
Classical wet mount method for observing live protists
SEM: Sample Preparation, Fixation and Staining of Protists
TEM: Sample Preparation, Fixation and Staining of Protists
Protargol staining technique for identification of soil protists.-FLUTAX Staining of cilliates
DNA extraction from trophozoites and cysts using manual protocol
DNA isolation from single ciliate isolate
Species identification through sequencing
DNA Barcoding techniques for Protist
Unequivocal identification through fluorescence in situ hybridization (FISH) technique
Isolation, DNA extraction, amplification, and gel electrophoresis of single-celled non-marine foraminifera (Rhizaria)
Isolation and cultivation and staining of Paramecium
Isolation and cultivation of Euglena.
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