9780128034897

0128034890

9780128034750

0128034750

Methods in cell biology ; v. 133

Front Cover; Methods in Cell Biology; Series Editors; Methods in Cell Biology: The Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology; Copyright; Dedication; Contents; Contributors; Preface; 1

Embryonic cell culture in zebrafish; 2

Cellular dissection of zebrafish hematopoiesis; 3

Second harmonic generation microscopy in zebrafish; 4

Imaging blood vessels and lymphatic vessels in the zebrafish; 5

An eye on light-sheet microscopy; 6

Single neuron morphology in vivo with confined primed conversion; 7

Visualizing retinoic acid morphogen gradients

8

Using fluorescent lipids in live zebrafish larvae: from imaging whole animal physiology to subcellular lipid tr ...9

Analysis of cilia structure and function in zebrafish; 10

Functional calcium imaging in zebrafish lateral-line hair cells; 11

Physiological recordings from the zebrafish lateral line; Volumes in Series; Index; A; B; C; D; E; F; G; H; I; K; L; M; N; O; P; Q; R; S; T; U; V; W; X; Y; Z; Back Cover; INTRODUCTION; 1. METHODS; CONCLUSION; REFERENCES; INTRODUCTION; 1. ZEBRAFISH HEMATOPOIESIS; 2. HEMATOPOIETIC CELL TRANSPLANTATION; 3. ENRICHMENT OF HSCS

4. IN VITRO CULTURE AND DIFFERENTIATION OF HEMATOPOIETIC PROGENITORSCONCLUSIONS; REFERENCES; INTRODUCTION; 1. MATERIALS; 2. METHODS; 3. NOTES; Acknowledgments; SUPPLEMENTARY DATA; REFERENCES; INTRODUCTION; 1. IMAGING VASCULAR GENE EXPRESSION; 2. NONVITAL BLOOD VESSEL AND LYMPHATIC VESSEL IMAGING; 3. VITAL IMAGING OF BLOOD AND LYMPHATIC VESSELS; CONCLUSION; REFERENCES; INTRODUCTION; HISTORY; 1. PRINCIPLE BEHIND SELECTIVE PLANE ILLUMINATION MICROSCOPY; 2. THE MICROSCOPE FOR YOUR SAMPLE OR THE SAMPLE FOR YOUR MICROSCOPE?; 3. DATA ACQUISITION AND HANDLING; 4. CHALLENGES AND PERSPECTIVES

AcknowledgmentREFERENCES; INTRODUCTION; 1. PHOTOCONVERTIBLE FLUORESCENT PROTEINS; 2. CONFINED PRIMED CONVERSION; 3. UNRAVELING SINGLE NEURON MORPHOLOGY WITH CONFINED PRIMED CONVERSION; CONCLUSION AND OUTLOOK; REFERENCES; INTRODUCTION; 1. CHALLENGES FOR MORPHOGEN GRADIENT STUDIES; 2. FEEDBACK ALLOWS RETINOIC ACID TO ACT AS A GRADED MORPHOGEN; 3. CYP26S AS KEY REGULATORS OF RETINOIC ACID GRADIENT FORMATION; 4. VISUALIZING THE RETINOIC ACID GRADIENT; 5. CRABPS AND RETINOIC ACID SIGNAL ROBUSTNESS; 6. SHARPENING BOUNDARIES OF GENE EXPRESSION IN RESPONSE TO RETINOIC ACID GRADIENTS

7. NOISE-BOTH GOOD AND BAD8. OTHER BOUNDARIES AND OTHER MORPHOGENS; CONCLUSIONS AND PERSPECTIVES; Acknowledgments; REFERENCES; INTRODUCTION; THE NEED FOR WHOLE ANIMAL STUDIES OF LIPID METABOLISM; 1. FORWARD GENETIC SCREENING WITH FLUORESCENT LIPIDS; 2. VISUALIZING LIPID METABOLISM USING BODIPY FATTY ACID ANALOGS; SUMMARY; Acknowledgments; REFERENCES; INTRODUCTION; 1. CILIA IN ZEBRAFISH ORGANS; 2. ANALYTICAL TOOLS FOR CILIA MORPHOLOGY AND MOTILITY; 3. PHENOTYPES OF CILIA MUTANTS IN ZEBRAFISH; 4. FUTURE DIRECTIONS; Acknowledgments; REFERENCES; INTRODUCTION

Zebra danio > Physiology.

Zebra danio > Development.

Cytology > Methodology.