Books+ Search Results
Cardiac Gene Expression Methods and Protocols
Title
Cardiac Gene Expression [electronic resource] : Methods and Protocols / edited by Jun Zhang, Gregg Rokosh.
ISBN
9781597450300
Publication
Totowa, NJ : Humana Press, 2007.
Physical Description
1 online resource (XII, 366 p.) 66 illus., 1 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function. Section I, Cardiac Gene Expression Profiling: The Global Perspective, discusses several different approaches to examining, identifying, and analyzing changes in transcriptome gene expression. Section II, Cardiac Gene Regulation: Gene-Specific mRNA Measurement in the Myocardium, outlines more sensitive and gene-targeted expression methods. Section III, Cardiac Gene Regulation: Promoter Characterization in the Myocardium, provides protocols for the study of underlying gene regulation mechanisms by focusing on the interaction of transcription factors with their cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome and other model genomes. The concluding section, Gene Overexpression and Targeting in the Myocardium, highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
August 31, 2018
Series
Methods in Molecular Biology, 366
Contents
Cardiac Gene Expression
Microarray Analysis of Gene Expression in Murine Cardiac Graft Infiltrating Cells
Expression Profiling Using Affymetrix GeneChip® Probe Arrays
Serial Analysis of Gene Expression (SAGE)
Functional Genomics by cDNA Subtractive Hybridization
Statistical Methods in Cardiac Gene Expression Profiling
Cardiac Gene Regulation
Measurement of Cardiac Gene Expression by Reverse Transcription Polymerase Chain Reaction (RT-PCR)
Quantitative (Real-Time) RT-PCR in Cardiovascular Research
RNase Protection Assay for Quantifying Gene Expression Levels
In Situ Hybridization
Cardiac Gene Regulation
Characterization of cis-Regulatory Elements and Transcription Factor Binding
Mapping Transcriptional Start Sites and In Silico DNA Footprinting
Characterization of Cardiac Gene Promoter Activity
In Silico Assessment of Regulatory cis-Elements and Gene Regulation
Comparative Genomics
Developing Computational Resources in Cardiac Gene Expression
Cardiac Single Nucleotide Polymorphisms
In Silico Analysis of SNPs and Other High-Throughput Data
Discovery and Identification of Sequence Polymorphisms and Mutations with MALDI-TOF MS
Gene Overexpression and Targeting in the Myocardium
Conditional Targeting
Cardiomyocyte Preparation, Culture, and Gene Transfer
Adeno-Associated Viral Vector-Delivered Hypoxia-Inducible Gene Expression in Ischemic Hearts
Lentivirus-Mediated Gene Expression.
Also listed under
Bookmark As
Citation
