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Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants
Title
Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants [electronic resource] / edited by S. Mohan Jain, Praveen K. Saxena.
ISBN
9781603272872
Publication
Totowa, NJ : Humana Press : Imprint: Humana Press, 2009.
Physical Description
1 online resource (XVIII, 350 p.) 113 illus.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the very survival of various species are of the utmost importance. In Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, expert researchers provide detailed, step-by-step protocols for the establishment of in vitro cultures of key medicinal plants, their mass multiplication in a controlled environment, and step-wise secondary metabolite analysis, genetic transformation, large-scale metabolite production in a bioreactor, and molecular markers. In addition, many of these protocols will provide a basis for much needed efforts of in vitro germplasm conservation or cryopreservation of medicinal plant species at the brink of extinction as well as efforts to protect them from the adverse impact of rapid climatic changes. As a volume in the Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants is an ideal resource for scientists endeavoring to continue the research on this exciting natural branch of medicine.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
August 31, 2018
Series
Methods in Molecular Biology, Methods and Protocols, 547
Contents
Micropropagation and Chemical Analysis
Establishment of Adventitious Root Cultures of Echinacea purpurea for the Production of Caffeic Acid Derivatives
In Vitro Propagation of Rauwolfia serpentina Using Liquid Medium, Assessment of Genetic Fidelity of Micropropagated Plants, and Simultaneous Quantitation of Reserpine, Ajmaline, and Ajmalicine
Medicinal Properties, In Vitro Protocols and Secondary Metabolite Analyses of Scots Pine
Saussurea medusa Cell Suspension Cultures for Flavonoid Production
Large-scale In Vitro Multiplication of Crataeva nurvala
Bilberry In Vitro Protocols and Analyses of Phenolic Compounds
In Vitro Propagation of Two Tuberous Medicinal Plants: Holostemma ada-kodien and Ipomoea mauritiana
In Vitro Production of Gymnemic Acid from Gymnema sylvestre (Retz) R. Br. Ex Roemer and Schultes Through Callus Culture Under Abiotic Stress Conditions
Establishment of Plant Regeneration and Cryopreservation System from Zygotic Embryo-Derived Embryogenic Cell Suspension Cultures of Ranunculus kazusensis
In Vitro Culture and Secondary Metabolite Isolation in Bryophytes
Micropropagation and In Vitro Conservation of Vanilla (Vanilla planifolia Andrews)
Protocol for In Vitro Regeneration and Marker Glycoside Assessment in Swertia chirata Buch-Ham
Protocols for Establishment of an In Vitro Collection of Medicinal Plants in the Genus Scutellaria
Protocols for In Vitro Culture and Phytochemical Analysis of Phyllanthus Species (Euphorbiaceae)
In Vitro Clonal Propagation of Asparagus racemosus, a High Value Medicinal Plant
Micropropagation of Penthorum chinenseThrough Axillary Buds
Transgenic Approaches
Spontaneous Plant Regeneration and Production of Secondary Metabolites from Hairy Root Cultures of Centaurium erythraea Rafn
Transgenic Hypericum perforatum
Agrobacterium-Mediated Transformation of Ruta graveolens L
Gene Expression Profiling in Taxus baccata L. Seedlings and Cell Cultures
Catapol Production in Chinese Foxglove (Rehmannia glutinosa Libos.) Hairy Roots Transformed with Agrobacterium rhizogenes ATCC15834
Molecular Markers and Microsatellites
Identification of Medicinal Plants and Plant Sequences: Multiplexed MLPA Assay
Isolation of Microsatellites from Catharanthus roseus (L.) G. Don Using Enriched Libraries
Biotransformation, Bioreactors, and Metabolomics
Production of Cinnamyl Glycosides in Compact Callus Aggregate Cultures of Rhodiola rosea Through Biotransformation of Cinnamyl Alcohol
Spearmint Plantlet Culture System as a Means to Study Secondary Metabolism
Bioreactor Production of Secondary Metabolites from Cell Cultures of Periwinkle and Sandalwood
Camptothecin Production by In Vitro Cultures and Plant Regeneration in Ophiorrhiza Species
Metabolomic Analysis of Ocotea odorifera Cell Cultures: A Model Protocol for Acquiring Metabolite Data
The Production of 9-methoxycanthin-6-one from Callus Cultures of (Eurycoma longifolia Jack) Tongkat Ali
Altered Gravity and Biotechnology
Plant Secondary Metabolism in Altered Gravity
The Role of Biotechnology in the Production of the Anticancer Compound Podophyllotox.
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