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Cystic Fibrosis Diagnosis and Protocols, Volume II: Methods and Resources to Understand Cystic Fibrosis
Title
Cystic Fibrosis [electronic resource] : Diagnosis and Protocols, Volume II: Methods and Resources to Understand Cystic Fibrosis / edited by Margarida D. Amaral, Karl Kunzelmann.
ISBN
9781617791208
Publication
Totowa, NJ : Humana Press, 2011.
Physical Description
1 online resource (XIV, 384 p.)
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
Despite the many milestones in cystic fibrosis (CF) research, progress toward curing the disease has been slow, and it is increasingly difficult to grasp and use the already wide and still growing range of diverse methods currently employed to study CF so as to understand it in its multidisciplinary nature. Cystic Fibrosis: Diagnosis and Protocols aims to provide the CF research community and related researchers with a very wide range of high-quality experimental tools, as an easy way to grasp and use classical and novel methods applied to cystic fibrosis. Volume II: Methods and Resources to Understand Cystic Fibrosis focuses on pathophysiology, Omics approaches, and a variety of key resources recently made available for CF research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Cystic Fibrosis: Diagnosis and Protocols will provide readers with optimal working tools to address pressing questions in the best technical way, while helping all of us, as a research and clinical community, to move faster hand-in-hand toward unravelling the secrets of this challenging disorder and cure it.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
August 31, 2018
Series
Methods in Molecular Biology, Methods and Protocols, 742
Contents
Introduction to Part I: Overview of Approaches to Study Cystic Fibrosis Pathophysiology
Imaging CFTR Protein Localization in Cultured Cells and Tissues
CFTR Regulation of Epithelial Sodium Channel
Methods for Evaluating Inflammation in Cystic Fibrosis
Methods for ASL Measurements and Mucus Transport Rates in Cell Cultures
Measurement of Fluid Secretion from Intact Airway Submucosal Glands
Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibrosis Human Airway Epithelia
Identification and Quantification of Mucin Expression
Methods to Classify Bacterial Pathogens in Cystic Fibrosis
Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells
Introduction to Part II: Omics in the Biology of Cystic Fibrosis
Microarray mRNA Expression Profiling to Study Cystic Fibrosis
Quantitative Differential Proteomics of Cystic Fibrosis Cell Models by SILAC (Stable Isotope Labelling in Cell Culture)
Application of Mass Spectrometry to Study Proteomics and Interactomics in Cystic Fibrosis
Functional Genomics Assays to Study CFTR Traffic and ENaC Function
New Lipidomic Approaches in Cystic Fibrosis
Introduction to Part III: Resources for CFTR Research
Primary Epithelial Cell Models for Cystic Fibrosis Research
Comparative Biology of Cystic Fibrosis Animal Models
CFTR Folding Consortium: Methods Available for Studies of CFTR Folding and Correction
Evaluation of the Disease Liability of CFTR Variants.
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